Side population cells expressing ABCG2 in human adult dental pulp tissue

被引：38
作者：
Honda, M. J.
Nakashima, F.
Satomura, K.
Shinohara, Y.
Tsuchiya, S.
Watanabe, N.
Ueda, M.
机构：
[1] Univ Tokyo, Inst Med Sci, Div Stem Cell Engn, Minato Ku, Tokyo 1088639, Japan
[2] Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Oral & Maxillofacial Surg, Tokushima 770, Japan
[3] Univ Tokyo, Inst Med Sci, FACS Core Lab, Tokyo, Japan
[4] Nagoya Univ, Grad Sch Med, Dept Oral & Maxillofacial Surg, Aichi, Japan
来源：
INTERNATIONAL ENDODONTIC JOURNAL | 2007年 / 40卷 / 12期
关键词：
ABCG2; dental stem cells; Hoechst; 33342; human dental pulp; odontoblast differentiation; side population;
D O I：
10.1111/j.1365-2591.2007.01301.x
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Aim To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue. Methodology Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT-PCR analyses. Results SP cells that express ABCG2, Nestin, Notch-1 and alpha-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disapeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured osteogenic medium. Conclusion Human adult dental pulp tissue contains SP cells that diferentiate into odontoblast-like cells.
引用
收藏
页码：949 / 958
页数：10
相关论文
共 53 条
[1]   Nestin expression in embryonic and adult human teeth under normal and pathological conditions [J].
About, I ;
Laurent-Maquin, D ;
Lendahl, U ;
Mitsiadis, TA .
AMERICAN JOURNAL OF PATHOLOGY, 2000, 157 (01) :287-295
[2]   Characterization of α-smooth muscle actin positive cells in mineralized human dental pulp cultures [J].
Alliot-Licht, B ;
Hurtrel, D ;
Gregoire, M .
ARCHIVES OF ORAL BIOLOGY, 2001, 46 (03) :221-228
[3]   Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures [J].
Alliot-Licht, B ;
Bluteau, G ;
Magne, D ;
Lopez-Cazaux, S ;
Lieubeau, B ;
Daculsi, G ;
Guicheux, J .
CELL AND TISSUE RESEARCH, 2005, 321 (03) :391-400
[4]   Myogenic specification of side population cells in skeletal muscle [J].
Asakura, A ;
Seale, P ;
Girgis-Gabardo, A ;
Rudnicki, MA .
JOURNAL OF CELL BIOLOGY, 2002, 159 (01) :123-134
[5]   Uno, nessuno e centomila:: Searching for the identity of mesodermal progenitors [J].
Bianco, P ;
Cossu, G .
EXPERIMENTAL CELL RESEARCH, 1999, 251 (02) :257-263
[6]  
Bleicher F, 2001, Adv Dent Res, V15, P30
[7]   α-smooth-muscle actin in and contraction of porcine dental pulp cells [J].
Brock, DP ;
Marty-Roix, R ;
Spector, M .
JOURNAL OF DENTAL RESEARCH, 2002, 81 (03) :203-208
[8]   Expression of the small leucine-rich proteoglycan osteoadherin/osteomodulin in human dental pulp and developing rat teeth [J].
Buchaille, R ;
Couble, ML ;
Magloire, H ;
Bleicher, F .
BONE, 2000, 27 (02) :265-270
[9]   Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells [J].
Budak, MT ;
Alpdogan, OS ;
Zhou, MY ;
Lavker, RM ;
Akinci, MAM ;
Wolosin, JM .
JOURNAL OF CELL SCIENCE, 2005, 118 (08) :1715-1724
[10]   ABC transporters as phenotypic markers and functional regulators of stem cells [J].
Bunting, KD .
STEM CELLS, 2002, 20 (01) :11-20
123456