Different nitrogen (N) fractions from 14 sediments from the shallow lakes in the middle and lower reaches of Yangtze River area before and after N release experiments were investigated, and the content of different N fractions, and contribution of different N fractions to the N released from sediments were also studied. Ion-exchangeable form (IEF-N), carbonate form (CF-N), iron-manganese oxide form (IMOF-N) and organic matter-sulfide form (OSF-N) accounted for 2.72 similar to 17.67%, 0.47 similar to 4.43%, 1.1 similar to 3.49% and 31.05 to 71.61% to total N, respectively. The N released was higher than 50% from IEF-N, approximately 35% from OSF-N, 6 and 8% from CF-N and IMOF-N on the average. Approximately 27.32 similar to 70.02% of IEF-N, 10.37 similar to 32.11% of CF-N, 11.37 similar to 33.43% IMOF-N and 2.02 similar to 8.19% OSF-N were released. For the sediments that were slightly polluted, IEF-N was the main N fraction that may be released and its contribution to total N released was more than 63.07%, for the sediments that TN was higher than 3,540.27 mg center dot kg(-1), OSF-N would become the main N fraction that can be released and its contribution to total N released was more than 45%.