The role of cell cycle-dependent neuropathy target esterase in cell proliferation

被引:7
作者
Chang, Ping-An [1 ,2 ]
Chen, Yu-Ying [1 ,3 ]
Qin, Wen-Zhen [1 ]
Long, Ding-Xin [2 ]
Wu, Yi-Jun [2 ]
机构
[1] Chongqing Univ Posts & Telecommun, Coll Bio Informat, Key Lab Mol Biol, Chongqing 400065, Peoples R China
[2] Chinese Acad Sci, Inst Zool, Mol Toxicol Lab, Beijing 100080, Peoples R China
[3] Xiamen Univ, Hosp Xiamen 1, Dept Neurosurg, Xiamen 361003, Peoples R China
基金
中国国家自然科学基金;
关键词
Neuropathy target esterase; Cell cycle; Cell proliferation; Short hairpin RNA; The ubiquitin-proteasome pathway; NEUROTOXIC ESTERASE; PHOSPHATIDYLCHOLINE; IDENTIFICATION; PROTEIN; BRAIN; GLYCEROPHOSPHOCHOLINE; EXPRESSION; GENE;
D O I
10.1007/s11033-010-0085-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neuropathy target esterase (NTE) is a novel phospholipase B and plays a role in phospholipid homeostasis. Although over-expression of NTE inhibits cell division, the role of NTE in cell proliferation is still unknown. In the current study, we firstly used synchronous HeLa cells to study the expression profile of NTE during the cell cycle. NTE protein and activity are regulated during the cell cycle with highest level at G(1) and lowest at G(2)/M phase. However, NTE mRNA levels are constant during the cell cycle. The role of NTE in cell proliferation was investigated by short hairpin RNA (shRNA) to suppress the expression of NTE. Knockdown of NTE significant down-regulated of NTE expression and reduced the glycerophosphocholine level. However, suppression of NTE did not affect phosphatidylcholine content or cell cycle progression. In addition, NTE was demonstrated to be degraded by the ubiquitin-proteasome pathway. These results suggested for the first time that NTE is a cell cycle-dependent protein, but is not essential for cell proliferation, and the ubiquitin-mediated proteolysis may be involved in the regulation of NTE during the cell cycle.
引用
收藏
页码:123 / 130
页数:8
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