CRISPR/Cas9-Based Split Fluorescent Protein Tagging

被引：2

作者：

Kesavan, Gokul ^{[1
]}

Machate, Anja ^{[1
]}

Brand, Michael ^{[1
]}

机构：

[1] Tech Univ Dresden, Ctr Regenerat Therapies TU Dresden, Fetscherstr 105, D-01307 Dresden, Germany

来源：

ZEBRAFISH | 2021年 / 18卷 / 06期

关键词：

split fluorescent protein; CRISPR; Cas9; knock-in; genetically encoded fluorescent protein tags;

D O I：

10.1089/zeb.2021.0031

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genetically encoded fluorescent tags such as green fluorescent protein fused to protein have revolutionized cell biology as they permit high-resolution protein imaging in live systems. Split fluorescent proteins, with a small fragment of 16 amino acids, can be inserted in the coding sequence to label proteins. We demonstrate successful integration of two bright and fast maturing split fluorescent proteins, mNeon green and sfCherry2, in zebrafish, and show that they are suitable for live imaging, including time-lapse series, and that they have a high signal-to-noise ratio. Furthermore, we show that CRISPR/Cas9 can be used to generate fluorescently tagged proteins in vivo.

引用

页码：369 / 373

页数：5

共 13 条

[11] Split Green Fluorescent Proteins: Scope, Limitations, and Outlook [J].