Sodium alginate potentiates antioxidants, cryoprotection and antibacterial activities of egg yolk extender during semen cryopreservation in buffalo

被引:22
作者
Kumar, Pradeep [1 ]
Pawaria, Shikha [1 ]
Dalal, Jasmer [1 ,2 ,3 ]
Ravesh, Suman [1 ]
Bharadwaj, Sonam [1 ,4 ]
Jerome, A. [1 ]
Kumar, Dharmendra [1 ]
Jan, Mustafa H. [1 ]
Yadav, P. S. [1 ]
机构
[1] ICAR Cent Inst Res Buffaloes, Anim Physiol & Reprod Div, Hisar 125001, Haryana, India
[2] Lala Lajpat Rai Univ Vet & Anim Sci, Dept Vet Gynaecol, Hisar, Haryana, India
[3] Lala Lajpat Rai Univ Vet & Anim Sci, Dept Obstet, Hisar, Haryana, India
[4] ICAR Natl Dairy Res Inst, Karnal, Haryana, India
关键词
Buffalo; Sperm; Cryopreservation; Fertility; Free radicals; OXIDATIVE STRESS; BUBALUS-BUBALIS; BOAR SPERMATOZOA; SEMINAL PLASMA; WATER-BUFFALO; OLIGOSACCHARIDES; CAPACITATION; ACID; MICROENCAPSULATION; FREEZABILITY;
D O I
10.1016/j.anireprosci.2019.106166
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The study was conducted to determine effects of sodium alginate on sperm during cryopreservation. Each ejaculate (n = 20) of five buffalo bulls (3-5 years) were divided into six equal fractions and diluted using egg yolk based extender supplemented with different concentrations of sodium alginate and cryopreserved. Frozen-thawed semen samples were evaluated using the CASA, hypo-osmotic swelling test, cervical mucus penetration capacity test, and chlortetracycline fluorescence assay (CTC). Phosphorylation of tyrosine containing proteins and malondialdehyde concentration of sperm membrane were evaluated using immunoblotting and thiobarbituric acid reactive substance assay respectively. The semen extender's anioxidative capacities were estimated by conducting 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays, metal chelating capacity by assessing ferrozine and antibacterial capacity using agar plate methods. Supplementation of sodium alginate in extender improved sperm longevity, plasma membrane integrity as well as capacity to transit through the cervical mucus. Supplementation of extender with sodium alginate minimises the phase transition of sperm membranes and phosphorylation of tyrosine containing proteins during cryopreservation. Malondialdehyde concentration of sperm was less in sodium alginate-treated sperm as compared with control samples. The results indicated that sodium alginate increased antioxidant capacity of semen extender. Supplementation with sodium alginate also improved the metal chelating capacity and antibacterial properties of the extender. In conclusion, supplementation of extender with sodium alginate enhances free radical scavenging, metal reduction and chelating capacities to protect sperm during cryopreservation.
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页数:10
相关论文
共 59 条
[1]   Antiviral Potential of Algae Polysaccharides Isolated from Marine Sources: A Review [J].
Ahmadi, Azin ;
Moghadamtousi, Soheil Zorofchian ;
Abubakar, Sazaly ;
Zandi, Keivan .
BIOMED RESEARCH INTERNATIONAL, 2015, 2015
[2]   Oxidative stress and male reproductive biology [J].
Aitken, RJ ;
Baker, MA .
REPRODUCTION FERTILITY AND DEVELOPMENT, 2004, 16 (05) :581-588
[3]   CRYOPRESERVATION ALTERS THE CA(2+) FLUX OF BOVINE SPERMATOZOA [J].
BAILEY, JL ;
BUHR, MM .
CANADIAN JOURNAL OF ANIMAL SCIENCE, 1994, 74 (01) :45-51
[4]  
Bailey JL, 2000, J ANDROL, V21, P1
[5]   Oxidative stress, osmotic stress and apoptosis: Impacts on sperm function and preservation in the horse [J].
Ball, Barry A. .
ANIMAL REPRODUCTION SCIENCE, 2008, 107 (3-4) :257-267
[6]   Physical, mechanical and antibacterial properties of alginate film: Effect of the crosslinking degree and oregano essential oil concentration [J].
Benavides, Sergio ;
Villalobos-Carvajal, R. ;
Reyes, J. E. .
JOURNAL OF FOOD ENGINEERING, 2012, 110 (02) :232-239
[7]   A differential mechanism is involved during heparin- and cryopreservation-induced capacitation of bovine spermatozoa [J].
Cormier, N ;
Bailey, JL .
BIOLOGY OF REPRODUCTION, 2003, 69 (01) :177-185
[8]   A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo [J].
Dalal, Jasmer ;
Kumar, Pradeep ;
Chandolia, R. K. ;
Pawaria, Shikha ;
Rajendran, Rasika ;
Sheoran, Suman ;
Andonissamy, Jerome ;
Kumar, Dharmendra .
SCIENTIFIC REPORTS, 2019, 9 (1)
[9]   Influence of Escherichia coli on motility parameters of human spermatozoa in vitro [J].
Diemer, T ;
Weidner, W ;
Michelmann, HW ;
Schiefer, HG ;
Rovan, E ;
Mayer, F .
INTERNATIONAL JOURNAL OF ANDROLOGY, 1996, 19 (05) :271-277
[10]  
Eliaz I, 2007, FORSCH KOMPLEMENTMED, V14, P358, DOI [10.1159/000109829, 10.1159/0000109829]