The effects of glucosamine derivatives on equine articular cartilage degradation in explant culture

被引:46
作者
Fenton, JI
Chlebek-Brown, KA
Peters, TL
Caron, JP
Orth, MW
机构
[1] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Large Anim Clin Sci, E Lansing, MI 48824 USA
关键词
glucosamine; osteoarthritis; articular cartilage; equine;
D O I
10.1053/joca.1999.0320
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Objective: To determine whether glucosamine-3-sulfate, glucose-3-sulfate (control) and N-acetyl glucosamine inhibit experimentally induced degradation of equine articular cartilage explants similar to glucosamine HCl. Design: Articular cartilage was obtained from the antebrachio-carpal and middle joints of horses (2-8 years old) killed for reasons unrelated to lameness. Cartilage discs were harvested from the weight-bearing region of the articular surface and cultured. Media were exchanged daily and the recovered media stored at 4 degreesC. On days 1 and 2 lipopolysaccharide (LPS, 10 mug/ml) was added to induce cartilage degradation. To evaluate the effects of different sources of glucosamine ton an equal molar basis), varying concentrations of glucosamine HCl (0.25, 2.5, or 25 mg/ml), glucosamine-3-sulfate (0.304, 3.04, or 30.4 mg/ml), or N-acetyl-glucosamine (0.256, 2.56, or 25.6 mg/ml) were added to the cultures. The glucose-3-sulfate control was added at 0.3075, 3.075 or 30.75 mg/ml. Nitric oxide and proteoglycan released into conditioned media and tissue proteoglycan synthesis and total tissue PG content were measured as indicators of cartilage metabolism. Results: Glucosamine-3-sulfate consistently inhibited cartilage degradation in a manner similar to glucosamine HCl, while the effects of N-acetyl-glucosamine were highly variable and did not inhibit cartilage degradation. Glucose-3-sulfate did not inhibit cartilage degradation. Conclusion: Our results indicate that glucosamine sulfate also has the potential to prevent or reduce articular cartilage degradation similar to glucosamine HCl in vitro. The amine group at the carbon-2 position appears important for the effectiveness of the glucosamine derivative. The therapeutic value of N-acetyl-glucosamine remains questionable. (C) 2000 OsteoArthritis Research Society International.
引用
收藏
页码:444 / 451
页数:8
相关论文
共 50 条
  • [21] Ex Vivo Equine Cartilage Explant Osteoarthritis Model: A Metabolomics and Proteomics Study
    Anderson, James R.
    Phelan, Marie M.
    Foddy, Laura
    Clegg, Peter D.
    Peffers, Mandy J.
    JOURNAL OF PROTEOME RESEARCH, 2020, 19 (09) : 3652 - 3667
  • [22] Glycosaminoglycans (GAGs) determination in healthy and damaged equine articular cartilage
    Adarmes, Hector
    Donders, Leonardo
    Dorner, Cristobal
    Gonzalez, Ema
    Galleguillos, Marco
    AUSTRAL JOURNAL OF VETERINARY SCIENCES, 2017, 49 (02) : 129 - 133
  • [23] The effect of link peptide on proteoglycan synthesis in equine articular cartilage
    Dean, MF
    Lee, YW
    Dastjerdi, AM
    Lees, P
    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2003, 1622 (03): : 161 - 168
  • [24] High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation
    Clutterbuck, Abigail L.
    Smith, Julia R.
    Allaway, David
    Harris, Pat
    Liddell, Susan
    Mobasheri, Ali
    JOURNAL OF PROTEOMICS, 2011, 74 (05) : 704 - 715
  • [25] Comparison of glucose derivatives effects on cartilage degradation
    Phitak, Thanyaluck
    Pothacharoen, Peraphan
    Kongtawelert, Prachya
    BMC MUSCULOSKELETAL DISORDERS, 2010, 11
  • [26] Botulinum neurotoxin type A does not exert concentration-dependent effects on equine articular cartilage in vitro
    Mccarthy, Meghan B.
    Duesterdieck-Zellmer, Katja F.
    Larson, Maureen K.
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 2023, 84 (09)
  • [27] Equine joint disease in the light of new developments in articular cartilage research
    van Weeren, PR
    Brama, PAJ
    PFERDEHEILKUNDE, 2003, 19 (04): : 336 - +
  • [28] An in vitro model for the pathological degradation of articular cartilage in osteoarthritis
    Grenier, Stephanie
    Bhargava, Madhu M.
    Torzilli, Peter A.
    JOURNAL OF BIOMECHANICS, 2014, 47 (03) : 645 - 652
  • [29] Postmortem degradation of porcine articular cartilage
    Rogers, Christopher J.
    Clark, Kirsty
    Hodson, Barbara J.
    Whitehead, Michael P.
    Sutton, Raul
    Schmerer, Wera M.
    JOURNAL OF FORENSIC AND LEGAL MEDICINE, 2011, 18 (02) : 52 - 56
  • [30] Immunolocalization of cathepsin B in equine dyschondroplastic articular cartilage
    Hernandez-Vidal, G
    Jeffcott, LB
    Davies, ME
    VETERINARY JOURNAL, 1998, 156 (03) : 193 - 201