Cardiomyocyte-transistor-hybrids for sensor application

被引：85
作者：
Ingebrandt, S
Yeung, CK
Krause, M
Offenhäusser, A
机构：
[1] Forschungszentrum Julich, Inst Thin Films & Interfaces ISG2, D-52425 Julich, Germany
[2] Max Planck Inst Polymer Res, D-55128 Mainz, Germany
来源：
BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 7-8期
关键词：
field effect transistors; extracellular recording; dissociated cardiac myocytes; pharmacological biosensor;
D O I：
10.1016/S0956-5663(01)00170-1
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described on the basis of an equivalent circuit. This technique is sensitive enough to detect minute changes of the extracellular membrane voltage and has potential applications in drug screening. We show that known cardiac stimulants (isoproterenol, norepinephrine) and relaxants (verapamil, carbamylcholine) have characteristic effects on the heart cells in terms of the changes of beat frequencies in the absence or presence of corresponding agents. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：565 / 570
页数：6
相关论文
共 18 条
[11]   Field-effect transistor array for monitoring electrical activity from mammalian neurons in culture [J].
Offenhausser, A ;
Sprossler, C ;
Matsuzawa, M ;
Knoll, W .
BIOSENSORS & BIOELECTRONICS, 1997, 12 (08) :819-826
[12]   Development and application of cell-based biosensors [J].
Pancrazio, JJ ;
Whelan, JP ;
Borkholder, DA ;
Ma, W ;
Stenger, DA .
ANNALS OF BIOMEDICAL ENGINEERING, 1999, 27 (06) :697-711
[13]   RECORDING ACTION-POTENTIALS FROM CULTURED NEURONS WITH EXTRACELLULAR MICRO-CIRCUIT ELECTRODES [J].
PINE, J .
JOURNAL OF NEUROSCIENCE METHODS, 1980, 2 (01) :19-31
[14]   SEALING CULTURED INVERTEBRATE NEURONS TO EMBEDDED DISH ELECTRODES FACILITATES LONG-TERM STIMULATION AND RECORDING [J].
REGEHR, WG ;
PINE, J ;
COHAN, CS ;
MISCHKE, MD ;
TANK, DW .
JOURNAL OF NEUROSCIENCE METHODS, 1989, 30 (02) :91-106
[15]   Long-term recording system based on field-effect transistor arrays for monitoring electrogenic cells in culture [J].
Sprossler, C ;
Richter, D ;
Denyer, M ;
Offenhausser, A .
BIOSENSORS & BIOELECTRONICS, 1998, 13 (06) :613-618
[16]   Electrical recordings from rat cardiac muscle cells using field-effect transistors [J].
Sprössler, C ;
Denyer, M ;
Britland, S ;
Knoll, W ;
Offenhäusser, A .
PHYSICAL REVIEW E, 1999, 60 (02) :2171-2176
[17]   An array of Pt-tip microelectrodes for extracellular monitoring of activity of brain slices [J].
Thiébaud, P ;
Beuret, C ;
Koudelka-Hep, M ;
Bove, M ;
Martinoia, S ;
Grattarola, M ;
Jahnsen, H ;
Rebaudo, R ;
Balestrino, M ;
Zimmer, J ;
Dupont, Y .
BIOSENSORS & BIOELECTRONICS, 1999, 14 (01) :61-65
[18]   MINIATURE MICROELECTRODE ARRAY TO MONITOR BIOELECTRIC ACTIVITY OF CULTURED CELLS [J].
THOMAS, CA ;
SPRINGER, PA ;
OKUN, LM ;
BERWALDN.Y ;
LOEB, GE .
EXPERIMENTAL CELL RESEARCH, 1972, 74 (01) :61-&
12