Diosmin mitigates high glucose-induced endoplasmic reticulum stress through PI3K/AKT pathway in HK-2 cells

被引:9
作者
Deng, Jiuhong [1 ,2 ]
Zheng, Chao [1 ,3 ]
Hua, Zhou [4 ]
Ci, Haideng [5 ]
Wang, Guiying [6 ]
Chen, Lijing [7 ,8 ,9 ,10 ]
机构
[1] Wenzhou Med Univ, Chashan Higher Educ Pk, Wenzhou 325035, Zhejiang, Peoples R China
[2] Second Peoples Hosp Pingyang Cty, Dept Endocrinol, Wenzhou 325405, Zhejiang, Peoples R China
[3] Zhejiang Univ, Affiliated Hosp 2, Sch Med, Jiefang Rd 88, Hangzhou 310009, Zhejiang, Peoples R China
[4] Poeples Hosp Suichang Cty, Dept Nephrol, Lishui City 323300, Zhejiang, Peoples R China
[5] Jiande Hosp Tradit Chinese Med, Dept Endocrinol & Nephrol, Hangzhou 311600, Zhejiang, Peoples R China
[6] Shangyu Peoples Hosp Shaoxing, Shaoxing City 312300, Zhejiang, Peoples R China
[7] Huzhou Cent Hosp, Dept Nephrol, Huzhou City 313000, Zhejiang, Peoples R China
[8] Huzhou Univ, Affiliated Cent Hosp, Huzhou City 313000, Zhejiang, Peoples R China
[9] Affiliated Huzhou Hosp, Huzhou City 313000, Zhejiang, Peoples R China
[10] Zhejiang Univ, Sch Med, Huzhou City 313000, Zhejiang, Peoples R China
关键词
Diabetic nephropathy; Diosmin; High glucose; Endoplasmic reticulum stress; Phosphatidylinositol; 3-kinase; protein kinase-B pathway; UNFOLDED PROTEIN RESPONSE; TUBULAR EPITHELIAL-CELLS; DIABETIC-NEPHROPATHY; OXIDATIVE STRESS; RAT MODEL; APOPTOSIS; MECHANISMS; AUTOPHAGY;
D O I
10.1186/s12906-022-03597-y
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background Diosmin has been reported to treat diabetes, but its role in diabetic nephropathy (DN) remains unclear. This research investigated the mechanism by which diosmin alleviated high glucose (HG)-induced HK-2 cell injury. Methods First, we used CCK-8 to detect the effect of 0.1, 1, or 10 mu g/mL diosmin on the viability of HK-2 cells treated with normal glucose or HG. Next, we used flow cytometry, automatic biochemical analyzer, ELISA, immunofluorescence, and colorimetric assay kit to examine the apoptosis, oxidative stress, inflammatory factors, and Caspase-3 expression in HK-2 cells. Thereafter, we used the western blot and qRT-PCR to examine the expression of the endoplasmic reticulum stress-, oxidative stress-, inflammation-, apoptosis-, and autophagy, and PI3K/AKT pathway-related factors. Results Diosmin was non-cytotoxic to normal HK-2 cells and enhanced the HK-2 cell viability suppressed by HG. Meanwhile, diosmin restrained apoptosis, the contents of MDA, pro-inflammatory factors, and Caspase-3 but intensified the contents of SOD and CAT induced by HG. We further confirmed that diosmin blunted oxidative stress-, inflammation-, apoptosis-, and autophagy-related factors expression induced by HG via restraining the CHOP and GRP78 expressions. Further, we also discovered that PTEN level was restrained and the ratios of p-PI3K/PI3K and p-AKT/AKT were enhanced in HK-2 cells induced by HG, which was reversed by co-treatment of HG and diosmin. Conclusions Our study manifested that diosmin alleviated the HG-mediated endoplasmic reticulum stress injury in HK-2 cells via restraining the PI3K/AKT pathway.
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页数:10
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