Production and purification of lipase by a mutant strain of Rhizopus arrhizus

被引：14
|
作者：
Chattopadhyay, M
Banik, AK [1 ]
Raychaudhuri, S
机构：
[1] Univ Calcutta, Dept Chem Engn, Div Biochem Engn, Calcutta 700009, W Bengal, India
[2] Univ Calcutta, Dept Biophys Mol Biol & Genet, Calcutta 700009, W Bengal, India
来源：
FOLIA MICROBIOLOGICA | 1999年 / 44卷 / 01期
关键词：
Lipase; Lipase Activity; Lipase Production; Aziridine; Extracellular Lipase;
D O I：
10.1007/BF02816218
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Extracellular lipase production by Rhizopus arrhizus was increased by mutant selection from 130 to 670 mu mol FFA per mt per min using UV radiation and aziridine treatment. The produced lipase was purified 720-fold by ammonium sulfate fractionation and Sephadex G-100 gel filtration. The molar mass of the produced lipase was determined to be approximately 67 kDa which comigrated with bovine serum albumin in both a Sephadex G-100 column and SDS-PAGE.
引用
收藏
页码：37 / 40
页数：4
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