A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids

被引：2
作者：
Boto, Luis [1 ]
Cunha, Carina [1 ,2 ]
Doadrio, Ignacio [1 ]
机构：
[1] CSIC, Museo Nacl Ciencias Nat, Dept Biodiversidad & Biol Evolut, E-28006 Madrid, Spain
[2] Inst Univ Lisboa, Lisbon, Portugal
来源：
GENETICS AND MOLECULAR BIOLOGY | 2011年 / 34卷 / 03期
关键词：
microsatellites; hybridogenesis; Squalius alburnoides; CROSS-SPECIES AMPLIFICATION; FRESH-WATER FISH; POPULATION-STRUCTURE; HYBRID COMPLEX; DNA-SEQUENCES; USEFUL TOOLS; DINUCLEOTIDE; EVOLUTION; MARKERS; LOCI;
D O I：
10.1590/S1415-47572011005000023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new microsatellite locus (SAS1) for Squalius alburnoides was obtained through cloning by serendipity. The possible usefulness of this new species-specific microsatellite in genetic studies of this hybrid-species complex, was explored. The polymorphism exhibited by SAS1 microsatellite is an important addition to the set of microsatellites previously used in genetic studies in S. alburnoides complex, that mostly relied in markers described for other species. Moreover, the SAS1 microsatellite could be used to identify the parental genomes of the complex, complementing other methods recently described for the same purpose.
引用
收藏
页码：524 / 528
页数：5
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