Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation

被引：4

作者：

Cerdeira, J. ^{[1
]}

Castano, C. ^{[2
]}

Perez, J. F. ^{[1
]}

Marcos-Beltran, J. L. ^{[3
]}

Guerra, R. ^{[4
]}

Lopez-Fernandez, M. ^{[5
]}

Torija, E. ^{[6
]}

Rodriguez, A. ^{[7
]}

Martinez-Nevado, E. ^{[8
]}

Toledano-Diaz, A. ^{[2
]}

Sanchez-Calabuig, M. J. ^{[1
]}

Santiago-Moreno, J. ^{[2
]}

机构：

[1] UCM, Dept Med & Surg, Sch Vet Med, Madrid, Spain

[2] INIA CSIC, Dept Anim Reprod, Madrid, Spain

[3] Consejeria Desarrollo Rural & Recursos Nat Princi, Asturias, Spain

[4] Zool Cordoba, Cordoba, Spain

[5] Zool Guadalajara, Guadalajara, Spain

[6] Mundopk, Seville, Spain

[7] Ctr Fauna Irrecuperable Kuna Iber, Madrid, Spain

[8] ZooAquarium, Madrid, Spain

来源：

ANIMAL REPRODUCTION SCIENCE | 2021年 / 235卷

关键词：

Wolf; Cryopreservation; Vitrification; Sperm; SEMEN COLLECTION METHOD; EPIDIDYMAL SPERM; SPERMATOZOA; WILD; WOLVES; RAMS; TESTOSTERONE; DESLORELIN; CARNIVORES; FERTILITY;

D O I：

10.1016/j.anireprosci.2021.106887

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Sperm vitrification is a simple, inexpensive method that allows the cryopreservation of sperm in the field and for endangered species is a useful alternative to conventional freezing. The study, therefore, is focused on the suitability of vitrification for cryopreserving Iberian wolf sperm and utilizing plasma testosterone concentration as a marker for procedure efficacy. Sperm and blood samples were collected from 17 wolves. There were 14 samples suitable for cryopreservation (12 ejaculated and two epididymal). Immediately after collection, these samples were proportioned into two aliquots for conventional freezing using a Tris-citric acid-glucose based extender (TCG) or vitrification utilizing an animal protein free extender (HTF (R)). Vitrification occurred by directly plunging a sperm suspension into liquid nitrogen. Sperm were assessed for motility, membrane integrity, acrosomal status and DNA integrity before and after cryopreservation. With both techniques, there were similar post-thaw/warming results (P 0.05) with respect to progressive motility, kinetic variables VCL, VSL, VAP and BCF, DNA fragmentation, sperm membrane functionality and morphological abnormalities. Total motile sperm, progression ratios LIN, STR, and WOB, the ALH, sperm viability and sperm with intact membrane and acrosome were greater (P < 0.05) in the conventional frozen-thawed sperm than vitrified-warmed sperm. Plasma testosterone concentrations varied from 0.0 ng/mL to 7.7 ng/mL. For epididymal sperm, sperm motility and viability following thawing were greater in vitrified-warmed samples than conventionally-frozen samples; however, small sample numbers precluded statistical analysis. When considered together, these results indicate vitrification may be a possible alternative for wolf sperm cryopreservation.

引用

页数：9

共 64 条

[1]

Bertschinger HJ, 2001, J REP FER S, P275

[2] Slow and ultra-rapid freezing protocols for cryopreserving mouflon (Ovis musimon) and fallow deer (Dama dama) epididymal sperm [J].