Simultaneous increase of mycelial biomass and intracellular polysaccharide from Fomes fomentarius and its biological function of gastric cancer intervention

被引：51

作者：

Chen, Wei ^{[1
,2
]}

Zhao, Zhao ^{[1
]}

Li, Yongquan ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310035, Zhejiang, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2011年 / 85卷 / 02期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金; 国家高技术研究发展计划(863计划);

关键词：

Fomes fomentarius; Intracelluar polysaccharide; Desirability functions; Submerged culture; Gastric cancer; SUBMERGED CULTURE; NUTRITIONAL-REQUIREMENTS; DESIRABILITY FUNCTION; CORDYCEPS-SINENSIS; GANODERMA-LUCIDUM; CELLS; APOPTOSIS; OPTIMIZATION; GROWTH; PH;

D O I：

10.1016/j.carbpol.2011.02.035

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this work, the effects of submerged culture conditions and nutritional requirements on simultaneous production of mycelial biomass and intracellular polysaccharide (IPS) from a medicinal mushroom Fomes fomentarius were studied using desirability functions. Under the optimal culture condition, the production of mycelia and IPS reached 17.19 and 2.86 gl(-1), respectively in a 151 stirred tank bioreactor, which were about twice than that of the basal medium. Furthermore, the ethanol extract of mycelia (EEM) and IPS had a direct antiproliferative effect on human gastric cancer cell lines SGC-7901 and MKN-45 in a dose-dependent manner. In contrast, human normal gastric cell line GES-1 was less susceptible to EEM and IPS. These results suggest that F. fomentarius may represent a promising novel approach for gastric cancer intervention. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：369 / 375

页数：7

共 37 条

[11] Nutritional requirements of mycelial growth of Cordyceps sinensis in submerged culture [J].

Dong, CH ;

Yao, YJ .

JOURNAL OF APPLIED MICROBIOLOGY, 2005, 99 (03) :483-492

[12] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[13] Dai-kenchu-to, a Chinese herbal medicine, improves stasis of patients with total gastrectomy and jejunal pouch interposition [J].

Endo, Shunji ;

Nishida, Toshirou ;

Nishikawa, Kazuhiro ;

Nakajima, Kiyokazu ;

Hasegawa, Jun-ichi ;

Kitagawa, Toru ;

Ito, Toshinori ;

Matsuda, Hikaru .

AMERICAN JOURNAL OF SURGERY, 2006, 192 (01) :9-13

[14] Effect of initial pH on production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidum [J].

Fang, QH ;

Zhong, JJ .

PROCESS BIOCHEMISTRY, 2002, 37 (07) :769-774

[15]

Fullerton SA, 2000, MOL UROL, V4, P7

[16]

Garraway MO., 1984, FUNGAL NUTR PHYSL, P401

[17] GROWTH-MORPHOLOGY OF STREPTOMYCES-AKIYOSHIENSIS IN SUBMERGED CULTURE - INFLUENCE OF PH, INOCULUM, AND NUTRIENTS [J].

GLAZEBROOK, MA ;

VINING, LC ;

WHITE, RL .

CANADIAN JOURNAL OF MICROBIOLOGY, 1992, 38 (02) :98-103

[18] Selective induction of apoptosis in murine skin carcinoma cells (CH72) by an ethanol extract of Lentinula edodes [J].

Gu, YH ;

Belury, MA .

CANCER LETTERS, 2005, 220 (01) :21-28

[19] Comparative study of antioxidant activity and antiproliferative effect of hot water and ethanol extracts from the mushroom Inonotus obliquus [J].

Hu, Honghai ;

Zhang, Zhenya ;

Lei, Zhongfang ;

Yang, Yingnan ;

Sugiura, Norio .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2009, 107 (01) :42-48

[20] Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus [J].

Hwang, HJ ;

Kim, SW ;

Xu, CP ;

Choi, JW ;

Yun, JW .

JOURNAL OF APPLIED MICROBIOLOGY, 2003, 94 (04) :708-719

← 1 2 3 4 →