Simultaneous increase of mycelial biomass and intracellular polysaccharide from Fomes fomentarius and its biological function of gastric cancer intervention

被引：51
作者：
Chen, Wei [1 ,2 ]
Zhao, Zhao [1 ]
Li, Yongquan [1 ]
机构：
[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310035, Zhejiang, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2011年 / 85卷 / 02期
基金：
中国国家自然科学基金; 高等学校博士学科点专项科研基金; 国家高技术研究发展计划(863计划);
关键词：
Fomes fomentarius; Intracelluar polysaccharide; Desirability functions; Submerged culture; Gastric cancer; SUBMERGED CULTURE; NUTRITIONAL-REQUIREMENTS; DESIRABILITY FUNCTION; CORDYCEPS-SINENSIS; GANODERMA-LUCIDUM; CELLS; APOPTOSIS; OPTIMIZATION; GROWTH; PH;
D O I：
10.1016/j.carbpol.2011.02.035
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In this work, the effects of submerged culture conditions and nutritional requirements on simultaneous production of mycelial biomass and intracellular polysaccharide (IPS) from a medicinal mushroom Fomes fomentarius were studied using desirability functions. Under the optimal culture condition, the production of mycelia and IPS reached 17.19 and 2.86 gl(-1), respectively in a 151 stirred tank bioreactor, which were about twice than that of the basal medium. Furthermore, the ethanol extract of mycelia (EEM) and IPS had a direct antiproliferative effect on human gastric cancer cell lines SGC-7901 and MKN-45 in a dose-dependent manner. In contrast, human normal gastric cell line GES-1 was less susceptible to EEM and IPS. These results suggest that F. fomentarius may represent a promising novel approach for gastric cancer intervention. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：369 / 375
页数：7
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