A polymerase chain reaction assay for detection of virulent and attenuated strains of duck plague virus

被引：9

作者：

Xie, Liji ^{[1
]}

Xie, Zhixun ^{[1
]}

Huang, Li ^{[1
]}

Wang, Sheng ^{[1
]}

Huang, Jiaoling ^{[1
]}

Zhang, Yanfang ^{[1
]}

Zeng, Tingting ^{[1
]}

Luo, Sisi ^{[1
]}

机构：

[1] Guangxi Vet Res Inst, Guangxi Key Lab Vet Biotechnol, Dept Biotechnol, 51 Youai North Rd, Nanning 530001, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2017年 / 249卷

关键词：

Duck plague virus; Virulent strain; Attenuated strain; PCR; MEDIATED ISOTHERMAL AMPLIFICATION; ENTERITIS VIRUS; SEQUENCE; DEV; DNA;

D O I：

10.1016/j.jviromet.2017.08.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequence analysis of duck plague virus (DPV) revealed that there was a 528 bp (B fragment) deletion within the UL2 gene of DPV attenuated vaccine strain in comparison with field virulent strains. The finding of gene deletion provides a potential differentiation test between DPV virulent strain and attenuated strain based on their UL2 gene sizes. Thus we developed a polymerase chain reaction (PCR) assay targeting to the DPV UL2 gene for simultaneous detection of DPV virulent strain and attenuated strain, 827 bp for virulent strain and 299 bp for attenuated strain. This newly developed PCR for DPV was highly sensitive and specific. It detected as low as 100fg of DNA on both DPV virulent and attenuated strains, no same size bands were amplified from other duck viruses including duck paramyxovirus, duck tembusu virus, duck circovirus, Muscovy duck parvovirus, duck hepatitis virus type I, avian influenza virus and gosling plague virus. Therefore, this PCR assay can be used for the rapid, sensitive and specific detection of DPV virulent and attenuated strains affecting ducks.

引用

页码：66 / 68

页数：3

共 21 条

[1] An outbreak of duck viral enteritis (duck plague) in domestic muscovy ducks (Cairina moschata domesticus) in Illinois [J].