Cytotoxic and genotoxic effects of 4-methylimidazole on Allium cepa root tips

被引：0

作者：

Recep, Liman ^{[1
]}

机构：

[1] Usak Univ, Fac Arts & Sci, Mol Biol & Genet Dept, TR-64300 Usak, Turkey

来源：

RESEARCH JOURNAL OF BIOTECHNOLOGY | 2020年 / 15卷 / 05期

关键词：

4-Methylimidazole; Allium cepa ana-telophase test; Comet assay; Chromosome aberrations; Toxicity; MERISTEM CELLS; IN-VITRO; TOXICITY; ASSAY; AMES/SALMONELLA; ABERRATION; SATIVUM; MITOSIS; OXIDE; MODE;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

4-Methylimidazole (4-MeI or 4-MEI) is widely used chemical as cleaning and agricultural chemical, drugs, rubber, dyes and pigments. It is also formed in manufacturing process of class III or IV caramel. In this study, Allium cepa root tips were used for cytogenotoxic effects of 4-MeI by using Allium anatelophase and comet assays. Changes in mitotic index (M), mitotic phase frequencies, chromosome aberrations (CAs) and DNA damage were evaluated after Allium bulbs exposed to three different concentrations (25, 50 and 100 ppm) of 4-MeI at four different time intervals (24, 48, 72 and 96 h) for comparison with a negative (distilled water) and positive control (Methyl methane sulfonate, WS-10 ppm). 4-MeI was found cytotoxic by reducing root growth and MI and was also found genotoxic by increasing CAs and DNA damage. Additional molecular toxicological studies are necessary to verify the cyto-genotoxicity of 4-MeI on plants.

引用

页码：121 / 127

页数：7

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