The role of miR319a and its target gene TCP4 in the regulation of pistil development in Prunus mume

被引：11

作者：

Wang, Wanxu ^{[1
,2
]}

Shi, Ting ^{[1
]}

Ni, Xiaopeng ^{[1
]}

Xu, Yanshuai ^{[1
]}

Qu, Shenchun ^{[1
]}

Gao, Zhihong ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Hort, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing, Jiangsu, Peoples R China

来源：

GENOME | 2018年 / 61卷 / 01期

基金：

美国国家科学基金会;

关键词：

pistil development; microRNA; Prunus mume; TCP4; COMPUTATIONAL IDENTIFICATION; MICRORNAS; RNA; ARABIDOPSIS; BIOGENESIS; FLOWER; MIRNAS; REPRESSION; MECHANISM; GENOMICS;

D O I：

10.1139/gen-2017-0118

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The microRNAs (miRNAs) comprise a broad class of non-coding small endogenous RNAs that are associated with many biological processes through the regulation of target genes, such as leaf morphogenesis and polarity, biotic and abiotic stress responses, and root and flower development. We identified a miRNA that affects flower development, miR319a, in Prunus mume. The Pm-miR319a target, Pm-TCP4, was validated by 5'RACE. The higher expression of Pm-TCP4 in imperfect flowers showed that Pm-TCP4 might promote pistil abortion. Further experiments showed that Pm-miR319a negatively regulates the expression of Pm-TCP4 mRNAs and affected pistil development. Sixteen downstream genes of Pm-TCP4 related to flower development were predicted. Previous studies have shown that they have an impact on the development of pistils. In this study it was established that Pm-miR319a indirectly regulates the development of pistils by regulating its target gene Pm-TCP4.

引用

页码：43 / 48

页数：6

共 48 条

[1] Diversifying microRNA sequence and function [J].

Ameres, Stefan L. ;

Zamore, Phillip D. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2013, 14 (08) :475-488

[2] Biogenesis and function of rice small RNAs from non-coding RNA precursors [J].

Arikit, Siwaret ;

Zhai, Jixian ;

Meyers, Blake C. .

CURRENT OPINION IN PLANT BIOLOGY, 2013, 16 (02) :170-179

[3] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[4] Arabidopsis lox3 lox4 double mutants are male sterile and defective in global proliferative arrest [J].

Caldelari, Daniela ;

Wang, Gaoge ;

Farmer, Edward E. ;

Dong, Xinnian .

PLANT MOLECULAR BIOLOGY, 2011, 75 (1-2) :25-33

[5] Regulation of miR394 in Response to Fusarium oxysporum f. sp cepae (FOC) Infection in Garlic (Allium sativum L) [J].

Chand, Subodh K. ;

Nanda, Satyabrata ;

Joshi, Raj K. .

FRONTIERS IN PLANT SCIENCE, 2016, 7

[6] microRNA biogenesis and function in plants [J].

Chen, XM .

FEBS LETTERS, 2005, 579 (26) :5923-5931

[7]

Dalmay T, 2013, ESSAYS BIOCHEM, V54, P29, DOI [10.1042/bse0540029, 10.1042/BSE0540029]

[8] Computational Identification, Target Prediction, and Validation of Conserved miRNAs in Insulin Plant (Costus pictus D. Don) [J].

Das, Akan ;

Das, Purabi ;

Kalita, Mohan C. ;

Mondal, Tapan K. .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2016, 178 (03) :513-526

[9] Class I TCP-DELLA Interactions in Inflorescence Shoot Apex Determine Plant Height [J].

Daviere, Jean-Michel ;

Wild, Michael ;

Regnault, Thomas ;

Baumberger, Nicolas ;

Eisler, Herfried ;

Genschik, Pascal ;

Achard, Patrick .

CURRENT BIOLOGY, 2014, 24 (16) :1923-1928

[10] The miRNA156/157 recognition element in the 3' UTR of the Arabidopsis SBP box gene SPL3 prevents early flowering by translational inhibition in seedlings [J].

Gandikota, Madhuri ;

Birkenbihl, Rainer P. ;

Hoehmann, Susanne ;

Cardon, Guillermo H. ;

Saedler, Heinz ;

Huijser, Peter .

PLANT JOURNAL, 2007, 49 (04) :683-693

← 1 2 3 4 5 →