CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma

被引：60
作者：
Zhao, Bao-Cheng [2 ]
Wang, Zhen-Jun [2 ]
Mao, Wei-Zheng [1 ]
Ma, Hua-Chong [2 ]
Han, Jia-Gang [2 ]
Zhao, Bo [2 ]
Xu, Hui-Min [3 ]
机构：
[1] Qingdao Univ Med Coll, Qingdao Municipal Hosp, Dept Gen Surg, Qingdao 266071, Peoples R China
[2] Capital Med Univ, Beijing Chaoyang Hosp, Dept Gen Surg, Beijing 100020, Peoples R China
[3] Weifang Peoples Hosp, Dept Gen Surg, Weifang 261041, Shangdong Prov, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2011年 / 17卷 / 19期
基金：
中国国家自然科学基金;
关键词：
Gastric carcinoma; Chemokines; Stromal cell-derived factor-1; CXC chemokine receptor-4; Lymph node metastasis; CHEMOKINE RECEPTOR CXCR4; SQUAMOUS-CELL CARCINOMA; BREAST-CANCER METASTASIS; COLORECTAL-CANCER; DOWN-REGULATION; NITRIC-OXIDE; LUNG-CANCER; EXPRESSION; CCR7; ASSOCIATION;
D O I：
10.3748/wjg.v17.i19.2389
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Aim: To investigate the role of CXC chemokine receptor-4 (CXCR4) and stromal cell-derived factor-1 (SDF-1) in lymph node metastasis of gastric carcinoma. METHODS: In 40 cases of gastric cancer, expression of CXCR4 mRNA in cancer and normal mucous membrane and SDF-1 mRNA in lymph nodes around the stomach was detected using quantitative polymerase chain reaction (PCR) (TaqMan) and immunohistochemistric assay. SGC-7901 and MGC80-3 cancer cells were used to investigate the effect of SDF-1 on cell proliferation and migration. RESULTS: Quantitative reverse transcription PCR and immunohistochemistry revealed that the expression level of CXCR4 in gastric cancer was significantly higher than that in normal mucous membrane (1.6244 +/- 1.3801 vs 1.0715 +/- 0.5243, P < 0.05). The expression level of CXCR4 mRNA in gastric cancer with lymph node metastasis was also significantly higher than that without lymph node metastasis (0.823 +/- 0.551 vs 0.392 +/- 0.338, P < 0.05). CXCR4 expression was significantly related to poorly differentiated, high tumor stage and lymph node metastasis. Significant differences in the expression level of SDF-1 mRNA were found between lymph nodes in metastatic gastric cancer and normal nodes (0.5432 +/- 0.4907 vs 0.2640 +/- 0.2601, P < 0.05). The positive expression of SDF-1 mRNA in lymph nodes of metastatic gastric cancer was consistent with the positive expression of CXCR4 mRNA in gastric cancer (r = 0.776, P < 0.01). Additionally, human gastric cancer cell lines expressed CXCR4 and showed vigorous proliferation and migratory responses to SDF-1. AMD3100 (a specific CXCR4 antagonist) was also found to effectively reduce the migration of gastric cancer cells. CONCLUSION: The CXCR4/SDF-1 axis is involved in the lymph node metastasis of gastric cancer. CXCR4 is considered as a potential therapeutic target in the treatment of gastric cancer. (C) 2011 Baishideng. All rights reserved.
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页码：2389 / 2396
页数：8
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