Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update

被引:239
作者
Peres Rubio, Camila [1 ]
Hernandez-Ruiz, Josefa [2 ]
Martinez-Subiela, Silvia [1 ]
Tvarijonaviciute, Asta [1 ,3 ]
Joaquin Ceron, Jose [1 ]
机构
[1] Univ Murcia, Fac Vet Med, Interdisciplinary Lab Clin Anal Interlab UMU, Reg Campus Int Excellence,Campus Mare Nostrum, E-30100 Murcia, Spain
[2] Univ Murcia, Fac Biol, Dept Plant Biol Plant Physiol, Murcia, Spain
[3] Univ Autonoma Barcelona, Sch Vet, Dept Anim Med & Surg, Barcelona, Spain
关键词
Antioxidant activity; Canine serum; Human serum; Cupric; Ferric; Oxidative stress; Trolox; OXIDATIVE STRESS MARKERS; IN-VITRO; ASCORBIC-ACID; TOTAL OXIDANT; DNA-DAMAGE; PLASMA; LIMITATIONS; BIOMARKERS; VALIDATION; PARAMETERS;
D O I
10.1186/s12917-016-0792-7
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.
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页数:7
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