Expression of recombinant hepatitis B virus antigen HBsAg in transgenic plant callus

被引：1

作者：

Kawashima, CG

Babá, EH

Hansen, E

机构：

[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biotecnol, BR-28015620 Campos Dos Goytacazes, RJ, Brazil

[2] Univ Fed Ouro Preto, ICEB, NUPEB, Dept Ciencias Biol, Ouro Preto, MG, Brazil

来源：

PROTEIN AND PEPTIDE LETTERS | 2001年 / 8卷 / 02期

关键词：

D O I：

10.2174/0929866013409571

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coding sequence of the surface antigen HBsAg of hepatitis B virus was cloned into a plant expression vector under the control of cauliflower mosaic virus 35S promoter. Cotyledons, hypocotyls and roots of lettuce cultivar "Vitoria" were transformed by cocultivation with Agrobacterium tumefaciens harbouring the recombinant plasmid. Lettuce callus tissue grown on selective medium was analysed and the presence of the transgene confirmed by PCR Expression of recombinant protein HBsAg was confirmed by Western Blot.

引用

页码：97 / 100

页数：4

共 12 条

[1] Delivery of immunoprophylactics in transgenic plants - Current status [J].

Hansen, E ;

Kawashima, CG .

BIODRUGS, 2000, 13 (06) :381-390

[2] THE FUNCTIONAL-ORGANIZATION OF THE NOPALINE A TUMEFACIENS PLASMID PTIC58 [J].

HOLSTERS, M ;

SILVA, B ;

VANVLIET, F ;

GENETELLO, C ;

DEBLOCK, M ;

DHAESE, P ;

DEPICKER, A ;

INZE, D ;

ENGLER, G ;

VILLARROEL, R ;

VANMONTAGU, M ;

SCHELL, J .

PLASMID, 1980, 3 (02) :212-230

[3] Natural history of chronic hepatitis B and C [J].

Imperial, JC .

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 1999, 14 :S1-S5

[4] Public perception and the safety of immunization - Commentary [J].

Kane, MA .

VACCINE, 1998, 16 :S73-S75

[5] A plant-derived edible vaccine against hepatitis B virus [J].

Kapusta, J ;

Modelska, A ;

Figlerowicz, M ;

Pniewski, T ;

Letellier, M ;

Lisowa, O ;

Yusibov, V ;

Koprowski, H ;

Plucienniczak, A ;

Legocki, AB .

FASEB JOURNAL, 1999, 13 (13) :1796-1799

[6]

MASON HS, 1992, P NATL ACAD SCI USA, V92, P3358

[7] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

[8] HEPATITIS VIRUSES - CHANGING PATTERNS OF HUMAN-DISEASE [J].

PURCELL, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2401-2406

[9]

SCOLNICK EM, 1984, JAMA-J AM MED ASSOC, V251, P2812

[10] BIOLOGY OF HEPATITIS-B VIRUS [J].

TIOLLAIS, P ;

CHARNAY, P ;

VYAS, GN .

SCIENCE, 1981, 213 (4506) :406-411

← 1 2 →