Isolating adipose-derived mesenchymal stem cells from lipoaspirate blood and saline fraction

被引：100

作者：

Francis, Michael P. ^{[1
]}

Sachs, Patrick C. ^{[2
]}

Elmore, Lynne W. ^{[1
,4
]}

Holt, Shawn E. ^{[1
,2
,3
,4
]}

机构：

[1] Virginia Commonwealth Univ, Dept Pathol, Richmond, VA 23284 USA

[2] Virginia Commonwealth Univ, Dept Human & Mol Genet, Richmond, VA USA

[3] Virginia Commonwealth Univ, Dept Pharmacol & Toxicol, Richmond, VA USA

[4] Virginia Commonwealth Univ, Massey Canc Ctr, Richmond, VA USA

来源：

ORGANOGENESIS | 2010年 / 6卷 / 01期

关键词：

adipose; stem cell; mesenchymal; epidermal growth factor; regenerative medicine; BONE-MARROW; TISSUE;

D O I：

10.4161/org.6.1.10019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isolation of adipose-derived stem cells (ASCs) typically involves 8+ hours of intense effort, requiring specialized equipment and reagents. Here, we present an improved technique for isolating viable populations of mesenchymal stem cells from lipoaspirate saline fractions within 30 minutes. Importantly, the cells exhibit remarkable similarities to those obtained using the traditional isolation protocols, in terms of their multipotent differentiation potential and immunophenotype. Reducing the acquisition time of ASCs is critical for advancing regenerative medicine therapeutics, and our approach provides rapid and simple techniques for enhanced isolation and expansion of patient-derived mesenchymal stem cells.

引用

页码：11 / 14

页数：4

共 8 条

[1] Expression of a functional epidermal growth factor receptor on human adipose-derived mesenchymal stem cells and its signaling mechanism [J].

Baer, Patrick C. ;

Schubert, Ralf ;

Bereiter-Hahn, Juergen ;

Ploesser, Michaela ;

Geiger, Helmut .

EUROPEAN JOURNAL OF CELL BIOLOGY, 2009, 88 (05) :273-283

[2] A perivascular origin for mesenchymal stem cells in multiple human organs [J].

Crisan, Mihaela ;

Yap, Solomon ;

Casteilla, Louis ;

Chen, Chien-Wen ;

Corselli, Mirko ;

Park, Tea Soon ;

Andriolo, Gabriella ;

Sun, Bin ;

Zheng, Bo ;

Zhang, Li ;

Norotte, Cyrille ;

Teng, Pang-Ning ;

Traas, Jeremy ;

Schugar, Rebecca ;

Deasy, Bridget M. ;

Badylak, Stephen ;

Buehring, Hans-Joerg ;

Giacobino, Jean-Paul ;

Lazzari, Lorenza ;

Huard, Johnny ;

Peault, Bruno .

CELL STEM CELL, 2008, 3 (03) :301-313

[3] Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue [J].

Izadpanah, Reza ;

Trygg, Cynthia ;

Patel, Bindiya ;

Kriedt, Christopher ;

Dufour, Jason ;

Gimble, Jeffery M. ;

Bunnell, Bruce A. .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 99 (05) :1285-1297

[4] Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells [J].

Katz, AJ ;

Tholpady, A ;

Tholpady, SS ;

Shang, HL ;

Ogle, RC .

STEM CELLS, 2005, 23 (03) :412-423

[5] Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue [J].

Kern, Susanne ;

Eichler, Hermann ;

Stoeve, Johannes ;

Klueter, Harald ;

Bieback, Karen .

STEM CELLS, 2006, 24 (05) :1294-1301

[6] Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates [J].

Yoshimura, Kotaro ;

Shigeura, Tomokuni ;

Matsumoto, Daisuke ;

Sato, Takahiro ;

Takaki, Yasuyuki ;

Aiba-Kojima, Emiko ;

Sato, Katsujiro ;

Inoue, Keita ;

Nagase, Takashi ;

Koshima, Isao ;

Gonda, Koichi .

JOURNAL OF CELLULAR PHYSIOLOGY, 2006, 208 (01) :64-76

[7] Human adipose tissue is a source of multipotent stem cells [J].

Zuk, PA ;

Zhu, M ;

Ashjian, P ;

De Ugarte, DA ;

Huang, JI ;

Mizuno, H ;

Alfonso, ZC ;

Fraser, JK ;

Benhaim, P ;

Hedrick, MH .

MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (12) :4279-4295

[8] Multilineage cells from human adipose tissue: Implications for cell-based therapies [J].

Zuk, PA ;

Zhu, M ;

Mizuno, H ;

Huang, J ;

Futrell, JW ;

Katz, AJ ;

Benhaim, P ;

Lorenz, HP ;

Hedrick, MH .

TISSUE ENGINEERING, 2001, 7 (02) :211-228

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