Layered chitosan-collagen hydrogel/aligned PLLA nanofiber construct for flexor tendon regeneration

被引：116
作者：
Deepthi, S. [1 ]
Sundaram, M. Nivedhitha [1 ]
Kadavan, J. Deepti [1 ]
Jayakumar, R. [1 ]
机构：
[1] Amrita Univ, Amrita Vishwa Vidyapeetham, Amrita Inst Med Sci & Res Ctr, Amrita Ctr Nanosci & Mol Med, Kochi 682041, Kerala, India
来源：
CARBOHYDRATE POLYMERS | 2016年 / 153卷
关键词：
Chitosan-collagen hydrogel; PLLA nanofibers; Alginate; Tendon regeneration; Peritendinous adhesion; IN-VIVO DEGRADATION; CELL-ADHESION; BONE; SCAFFOLDS; ATTACHMENT; CARTILAGE; STIFFNESS; ALGINATE; INJURY; REPAIR;
D O I：
10.1016/j.carbpol.2016.07.124
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The aim of our study was to develop a tendon construct of electrospun aligned poly (L-lactic acid) (PLLA) nanofibers, to mimic the aligned collagen fiber bundles and layering PLLA fibers with chitosan-collagen hydrogel, to mimic the glycosaminoglycans of sheath ECM for tendon regeneration. The hydrogel coated electrospun membrane was rolled and an outer coating of alginate gel was given to prevent peritendinous adhesion. The developed constructs were characterized by SEM, FT-IR and tensile testing. Protein adsorption studies showed lower protein adsorption on coated scaffolds compared to uncoated scaffolds. The samples were proven to be non-toxic to tenocytes. The chitosan-collagen/PLLA uncoated scaffolds and alginate gel coated chitosan-collagen/PLLA scaffolds showed good cell proliferation. The tenocytes showed good attachment and spreading on the scaffolds. This study indicated that the developed chitosan-collagen/PLLA/alginate scaffold would be suitable for flexor tendon regeneration. (C) 2016 Elsevier Ltd. All rights reserved.
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收藏
页码：492 / 500
页数：9
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