Analysis of Length and Orientation of Microtubules in Wide-Field Fluorescence Microscopy

被引：0
|
作者：
Herberich, Gerlind [1 ]
Ivanescu, Anca [1 ]
Gamper, Ivonne [2 ]
Sechi, Antonio [2 ]
Aach, Til [1 ]
机构：
[1] Rhein Westfal TH Aachen, Inst Imaging & Comp Vis, Aachen, Germany
[2] Rhein Westfal TH Aachen, Dept Cell Biol, Inst Biomed Engn, Aachen, Germany
来源：
PATTERN RECOGNITION | 2010年 / 6376卷
关键词：
LISTERIA-MONOCYTOGENES; STEERABLE FILTERS; MOTILITY; DYNAMICS; CELL; CYTOSKELETON; DESIGN; ACTIN;
D O I：
暂无
中图分类号：
TP18 [人工智能理论];
学科分类号：
081104 ; 0812 ; 0835 ; 1405 ;
摘要：
In this paper we present a novel approach for the analysis of microtubules in wide-field fluorescence microscopy. Microtubules are flexible elongated structures and part of the cytoskeleton, a cytoplasmic scaffolding responsible for cell stability and motility. The method allows for precise measurements of microtubule length and orientation under different conditions despite a high variability of image data and in the presence of artefacts. Application of the proposed method to demonstrate the effect of the protein GAR22 on the rate of polymerisation of microtubules illustrates the potential of our approach.
引用
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页码：182 / +
页数：3
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