Protective Effects of Hesperetin Against Lipopolysaccharide-induced Acute Renal Injury in Rat

Objective: In this study, it was aimed to investigate the effects of hesperetin on renal transforming growth factor-beta 1 (TGF-beta 1) expression and apoptosis in rat lipopolysaccharide (LPS)-induced acute renal injury model. Methods: In the study, 18 adult male Wistar albino rats were used. Rats divided into three groups, respectively (n=6); control, LPS and LPS + hesperetin. Sepsis model was created with a singledose of LPS (Escherichia coli, O26: B6 serotype, Sigma-aldrich). LPS + hesperetin group was administered intragastrically with the aid of hesperetin oral gavage at a dose of 100 mg/kg, after LPS-induction. Twenty four hours after LPS administration, the rats were opened from the midline under ketamine-xylazine anesthesia and kidney tissue and cardiac blood were collected. Kidney tissue was examined with hematoxylin-eosin staining. TGF-beta 1 expression was determined by indirect immunohistochemical method. TUNEL method was used to determine renal apoptosis. Blood urea nitrogen (BUN) and creatinine levels in blood serum were determined using spectrophotometric methods. Results: Decreased histopathological changes, TGF-beta 1 expression and apoptosis were determined in the LPS + hesperetin group compared to the LPS group (p<0.05). In addition, a significant decrease in BUN and creatinine levels was observed in the LPS + hesperetin group compared to the LPS group (p<0.05). Conclusion: The data obtained show that in the LPS-induced rat sepsis model, hesperetin suppresses the expression of TGF-beta 1 in kidney tissue and provides a protective effect.