CONTINUOUS LACTATE MONITORING BY MICROSENSORS IN SPHEROID 3D TUMOR CELL CULTURES

被引：0

作者：

Weltin, A. ^{[1
]}

Hammer, S. ^{[1
]}

Kaminski, Y. ^{[2
]}

Klein, S. ^{[2
]}

Noor, F. ^{[2
]}

Kieninger, J. ^{[1
]}

Urban, G. A. ^{[1
]}

机构：

[1] Univ Freiburg, Dept Microsyst Engn IMTEK, Lab Sensors, Freiburg, Germany

[2] Univ Saarland, Inst Biomed Engn, Saarbrucken, Germany

来源：

2015 TRANSDUCERS - 2015 18TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS) | 2015年

关键词：

3D cell culture; spheroids; lactate; microsensors; monitoring; toxicology;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This paper reports the combination of an electrochemical metabolic monitoring system with a spheroid 3D tumor cell culture environment. Precise lactate production from single human hepatocyte spheroids was continuously monitored online over hours by electrochemical microsensors, also in combination with drug exposure. The approach enhances and simplifies the determination of metabolic rates from organotypic 3D cell cultures in drug discovery and toxicology.

引用

页码：1695 / 1698

页数：4

共 6 条

[1] Real-time In-situ Lactate Monitoring in 3D Multi-Cellular Spheroid Cultures by using Enzyme-Based Biosensors in Hanging Drop Networks
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[6] Cell culture monitoring for drug screening and cancer research: a transparent, microfluidic, multi-sensor microsystem
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[J]. LAB ON A CHIP, 2014, 14 (01) : 138 - 146

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