Monoclonal Antibody Targeting the HA191/199 Region of H1N1 Influenza Virus Mediates the Damage of Neural Cells

被引：3

作者：

Guo, Chun-Yan ^{[1
,2
]}

Feng, Qing ^{[1
,2
]}

Yan, Li-Ting ^{[1
,2
]}

Xie, Xin ^{[3
]}

Liang, Dao-Yan ^{[1
,2
]}

Li, Yan ^{[1
,2
]}

Feng, Yang-Meng ^{[1
,2
]}

Sun, Li-Jun ^{[1
,2
]}

Hu, Jun ^{[1
,2
]}

机构：

[1] Shaanxi Prov Peoples Hosp, Cent Lab, Xian 710068, Shaanxi, Peoples R China

[2] Shaanxi Prov Res Ctr Cell Immunol Engn & Technol, Xian 710068, Shaanxi, Peoples R China

[3] Northwest Univ, Coll Life Sci, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Xian 710069, Shaanxi, Peoples R China

来源：

BIOCHEMISTRY-MOSCOW | 2021年 / 86卷 / 11期

关键词：

influenza A virus; HA191/199; region; monoclonal antibodies (mAbs); neuronal cell damage;

D O I：

10.1134/S0006297921110109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vaccination is the most effective mean of preventing influenza virus infections. However, vaccination-induced adverse reactions of the nervous system, the causes of which are unknown, lead to concerns on the safety of influenza A vaccine. In this study, we used flow cytometry, cell ELISA, and immunofluorescence to find that H1-84 monoclonal antibody (mAb) against the191/199 region of the H1N1 influenza virus hemagglutinin (HA) protein binds to neural cells and mediates cell damage. Using molecular simulation software, such as PyMOL and PDB viewer, we demonstrated that the HA191/199 region maintains the overall structure of the HA head. Since the HA191/199 region cannot be removed from the HA structure, it has to be altered via introducing point mutations by site-directed mutagenesis. This will provide an innovative theoretical support for the subsequent modification the influenza A vaccine for increasing its safety.

引用

页码：1469 / 1476

页数：8

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