Gastrointestinal nematodes from buffalo in Minoufiya Governorate, Egypt with special reference to Bunostomum phlebotomum

Gastrointestinal nematodes cause massive economic losses as an important impediment to the development of animals around the world. This study aimed to investigate the microscopical diagnosis of nematode parasites of buffalo from Minoufiya, Egypt and molecular characterization of Bunostomum phlebotomum. We examined 390 fecal samples with floatation and fecal culture techniques to recognize different genera of nematodes. The results revealed B. phlebotomum (2.56%), Strongyloides papillosus (3.85%), Toxocara vitulorum (7.69%), Haemonchus sp. (1.28%), and Dictyocaulus viviparus (1.28%). The recovered eggs and larvae of nematodes were identified as well as the adults of B. phlebotomum. Age-wise, sex-wise, and seasonal prevalences of the recovered nematodes were recorded. Sequence analysis of the ITS-2 gene of B. phlebotomum was highly identical (99-100%) to sequences from Australia and China and occurred in the same clade with B. trigoncephalum. In conclusion, the study presented the coprological survey of gastrointestinal nematodes, and the genetic characterization of B. phlebotomum from Minoufiya Governorate, Egypt for the first time.