Hypoglycemic effect and mechanism of a pectic polysaccharide with hexenuronic acid from the fruits of Ficus pumila L. in C57BL/KsJdb/db mice

被引：97

作者：

Wu, Jianjun ^{[1
,2
]}

Chen, Miaomiao ^{[1
,2
]}

Shi, Songshan ^{[1
,2
]}

Wang, Huijun ^{[1
,2
]}

Li, Ning ^{[1
,2
]}

Su, Juan ^{[1
,2
]}

Liu, Ruimin ^{[1
,2
]}

Huang, Zhenlin ^{[1
,2
]}

Jin, Hong ^{[4
]}

Ji, Xueqing ^{[3
]}

Wang, Shunchun ^{[1
,2
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, MOE Key Lab Stand Chinese Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, SATCM Key Lab New Resources & Qual Evaluat Chines, Inst Chinese Mat Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China

[3] Shanghai Univ Tradit Chinese Med, LongHua Hosp, 725 South Wanping Rd, Shanghai 201203, Peoples R China

[4] Qing Dao Environm Monitoring Stn, 41 Yanan Rd, Qingdao 266003, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2017年 / 178卷

基金：

中国国家自然科学基金;

关键词：

Pectin; Polysaccharide; C57BL/KsJdb/db mice; Type 2 diabetes mellitus; Hypoglycemic effect; Hepatic glycogen; INSULIN-RESISTANCE; AMELIORATES HYPERGLYCEMIA; GLUCOSE; MODEL; ACTIVATION; EXPRESSION; PEPCK;

D O I：

10.1016/j.carbpol.2017.09.050

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, a particular pectic polysaccharide (FPLP) was extracted and purified from the fruits of Ficus pumila Linn. through boiling water extraction, alcohol precipitation, diethylaminoethyl-Sepharose Fast Flow chromatography and Superdex T G-75 gel filtration chromatography. Analysis of high-performance gel permeation chromatography, FTIR, GC-MS, methylation and 1D/2D NMR spectroscopy revealed that FPLP (Mw: 34.69 kDa) is a linear (1,4)-alpha-D-galacturonic acid binding 1.30% branched chain hexenuronic acid with 23.34% methyl esterification. Treatment with FPLP ameliorated hyperglycaemia in association with an improvement in hepatic glycogen metabolism in C57BL/KsJ db/db mice. The activation of IRS-1/PI3K/Akt/GSK3 beta/GS insulin signalling pathway and AMPK/GSK3 beta/GS signalling pathway and the regulation of glucokinase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase expressions involved in hepatic glycogenesis and glycogenolysis were considered the therapeutic mechanisms of FPLP. These results provide a new insight for investigating the effects of pectic polysaccharides on blood glucose control and suggest that FPLP is a promising nutraceutical for treatment of T2DM.

引用

页码：209 / 220

页数：12

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