MicroRNA-145 suppresses epithelial to mesenchymal transition in pancreatic cancer cells by inhibiting TGF-β signaling pathway

被引：18
作者：
Chen, Shaojun [1 ]
Xu, Junyi [2 ]
Su, Yao [3 ]
Hua, Li [1 ]
Feng, Chengjun [1 ]
Lin, Zhan [4 ]
Huang, Haixin [1 ]
Li, Yongqiang [5 ]
机构：
[1] Guangxi Med Univ, Dept Oncol, Affiliated Hosp 4, 1 Liushi Rd, Liuzhou 545005, Guangxi, Peoples R China
[2] Guangxi Med Univ, Dept Gen Surg, Affiliated Hosp 4, Liuzhou, Guangxi, Peoples R China
[3] Nanjing Zhongshan Biomed Translat Inst, Nanjing, Jiangsu, Peoples R China
[4] Yulin First Peoples Hosp, Dept Oncol, Yulin, Guangxi, Peoples R China
[5] Guangxi Med Univ, Dept Chemotherapy, Affiliated Tumor Hosp, 6 Binhu Rd, Nanning 530021, Guangxi, Peoples R China
来源：
JOURNAL OF CANCER | 2020年 / 11卷 / 09期
基金：
中国国家自然科学基金;
关键词：
miRNA-145; pancreatic cancer; EMT; metastasis; TGF- beta signaling; PROGRESSION; INVASION;
D O I：
10.7150/jca.34902
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
TGF-beta signaling plays a critical role in tumor progression and many approaches have been made to inhibit its functions. MicroRNA is one of the approaches that inhibit TGF-beta signaling and can be used as a promising treatment for cancer. This study explored the role of miRNA-145 in pancreatic cancer (PC) development. The expression of miRNA-145 in PC tissues and paired adjacent normal tissues was examined by qRT-PCR. The expression of miRNA-145 in PC cells and the ability of cell migration and invasion were detected both in vivo and in vitro. The results showed that miRNA-145 was down-regulated in PC tissues and PC cells. Increasing the expression of miRNA-145 in PC cells inhibited the TGF-beta signaling pathway and epithelial-mesenchymal transition (EMT) process. Scratch assay and transwell assay showed that miRNA-145 inhibited the migration and invasion in PC cells. In vivo experiments confirmed that miRNA-145 mimics delayed the growth of PC xenografts comparing with miRNA-145 inhibitor. Our results suggested that miRNA-145 can inhibit epithelial to mesenchymal transition (EMT) and tumor growth by suppressing TGF-beta signaling pathway. Thus, miRNA-145 could be a potential therapeutic for targeting TGF-beta signaling in PC treatment.
引用
收藏
页码：2716 / 2723
页数：8
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