Bioavailability evaluation of calcium, magnesium and zinc in Brazilian cheese through a combined model of in vitro digestion and Caco-2 cells

被引:15
作者
da Paixao Teixeira, Jose Luan [1 ]
Lima Pallone, Juliana Azevedo [1 ]
Delgado Andrade, Cristina [2 ]
Mesias, Marta [3 ]
Seiquer, Isabel [2 ]
机构
[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci & Nutr, St Monteiro Lobato 80, BR-13083862 Campinas, SP, Brazil
[2] CSIC, Dept Physiol & Biochem Anim Nutr, Estn Expt Zaidin, Granada 18100, Spain
[3] Inst Food Sci Technol & Nutr, ICTAN, CSIC, Jose Antonio Novais 10, Madrid 28040, Spain
基金
巴西圣保罗研究基金会;
关键词
Food analysis; Food composition; Cheese; Mineral bioaccessibility; Caco-2; cells; Furosine content; MAILLARD REACTION-PRODUCTS; ANTIOXIDANT PROPERTIES; DAIRY-PRODUCTS; AVAILABILITY; MILK; BIOACCESSIBILITY; ABSORPTION; MINERALS; ELEMENTS; CASEIN;
D O I
10.1016/j.jfca.2021.104365
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Cheese has important nutritional properties as a result of their protein, lipid and essential minerals content. This study analyzed the bioavailability of Ca, Mg and Zn in Brazilian cheeses, using the in vitro INFOGEST digestion method coupled with assays in Caco-2 cells. Furosine content (marker of the early Maillard reaction) was also evaluated. Two self-produced fresh cheeses, cow and goat Minas frescal; and two commercial matured goat cheeses, Blue and Pyramid, were analyzed. Mineral bioaccessibility after in vitro digestion was in the range of 44.4-74.7 %, 54.8-66.1 % and 18.2-38.7 % for Ca, Mg and Zn, respectively. Bioaccessibilty was significantly affected by the mineral and fat content level. The digested fresh cheeses (Minas frescal) showed the highest values of soluble Ca and Mg; and the Pyramid goat cheese presented the greatest Ca and Zn transport efficacy across Caco-2 cell monolayers. Fumsine could be a useful indicator to evaluate cheese ripeness and its indirect effect on the mineral availability.
引用
收藏
页数:9
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