Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis

被引:68
作者
Helps, C [1 ]
Reeves, N [1 ]
Egan, K [1 ]
Howard, P [1 ]
Harbour, D [1 ]
机构
[1] Univ Bristol, Dept Vet Clin Sci, Div Mol & Cellular Biol, Bristol BS40 5DU, Avon, England
关键词
D O I
10.1128/JCM.41.6.2734-2736.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.
引用
收藏
页码:2734 / 2736
页数:3
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