Evaluation of disk diffusion method compared to broth microdilution for antifungal susceptibility testing of 3 echinocandins against Aspergillus spp.

被引：12

作者：

Isabel Martos, Antonia ^{[1
]}

Martin-Mazuelos, Estrella ^{[1
]}

Romero, Ana ^{[1
]}

Serrano, Carmen ^{[1
]}

Gonzalez, Trinidad ^{[1
]}

Almeida, Carmen ^{[2
]}

Puche, Beatriz ^{[1
]}

Canton, Emilia ^{[3
]}

Peman, Javier ^{[3
]}

Espinel-Ingroff, Ana ^{[4
]}

机构：

[1] Valme Univ Hosp, Microbiol Serv, Seville, Spain

[2] Valme Univ Hosp, Biostat Serv, Seville, Spain

[3] La Fe Univ Hosp, Microbiol Serv, Valencia, Spain

[4] VCU Med Ctr, Richmond, VA USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2012年 / 73卷 / 01期

关键词：

Echinocandin; Aspergillus; Susceptibility testing; Disk diffusion; INVASIVE FUNGAL-INFECTIONS; IN-VITRO ACTIVITY; FILAMENTOUS FUNGI; AMPHOTERICIN-B; CASPOFUNGIN; ANIDULAFUNGIN; ITRACONAZOLE; POSACONAZOLE; VORICONAZOLE; MICAFUNGIN;

D O I：

10.1016/j.diagmicrobio.2012.01.006

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We tested the susceptibility of caspofungin, micafungin, and anidulafungin against Aspergillus spp. isolates by the new Clinical and Laboratory Standards Institute M51-A disk diffusion (DD) and the broth microdilution methods. A total of 65 clinical isolates of Aspergillus spp. were evaluated. The DD assay was performed on nonsupplemented Mueller-Hinton agar using caspofungin 2-mu g, micafungin 1-mu g, and anidulafungin 2-mu g disks. Echinocandin minimal effective concentrations (MECs) and inhibition zones (IZs) were read after 24 to 48 (A. terreus) h at 35 degrees C. Caspofungin MECs for all Aspergillus spp. strains tested were <= 0.25 mu g/mL; IZs were >= 15 mm for most species except for A. terreus (11-22 mm). Both micafungin and anidulafungin MECs were <= 0.015 mu g/mL, but micafungin IZs were >= 14 mm while anidulafungin IZs were >= 22 mm. As for caspofungin, the DD method could be a useful method for susceptibility testing of micafungin and anidulafungin against Aspergillus spp. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.

引用

页码：53 / 56

页数：4

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