Novel superparamagnetic iron oxide nanoparticles for tumor embolization application: Preparation, characterization and double targeting

被引：42

作者：

Chen, Xiaoli ^{[1
]}

Lv, Haiyan ^{[1
]}

Ye, Min ^{[1
]}

Wang, Shengyu ^{[1
]}

Ni, Erru ^{[1
]}

Zeng, Fanwei ^{[1
]}

Cao, Chang ^{[1
]}

Luo, Fanghong ^{[1
]}

Yan, Jianghua ^{[1
]}

机构：

[1] Xiamen Univ, Canc Res Ctr, Coll Med, Xiamen 361005, Fujian, Peoples R China

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2012年 / 426卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Magnetic nanoparticles; Superparamagnetic; o-Carboxymethylchitosan; Truncated tissue factor; Tumor targeting; FACTOR FUSION PROTEINS; TISSUE-FACTOR; MAGNETIC NANOPARTICLES; FE3O4; NANOPARTICLES; ANGIOGENESIS; MECHANISMS; SEPARATION; CHITOSAN; RECEPTOR; MUTANTS;

D O I：

10.1016/j.ijpharm.2012.01.043

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The goal of this study was to develop novel embolic nanoparticles for targeted tumor therapy with dual targeting: magnetic field-guided and peptide-directed targeting. The embolic nanoparticles SP5.2/tTF-OCMCs-SPIO-NPs were prepared by surface-modifying of superparamagnetic iron oxide nanoparticles (SPIO-NPs) with o-carboxymethylchitosans (OCMCs) and SP5.2/tTF (SP5.2: a peptide binding to VEGFR-1; tTF: truncated tissue factor) to improve their stability and to target over-expressing VEGFR-1 cells. The physicochemical characterization results showed that the OCMCs-SPIO-NPs have a spherical or ellipsoidal morphology with an average diameter of 10-20 nm. And they possess magnetism with a saturation magnetization of 66.1 emu/g, negligible coercivity and remanence at room temperature. In addition, the confocal microscopy, Prussian blue staining and FX activation analysis respectively demonstrated the peptide-directed targeting, magnetic field-guided targeted and blood coagulation activity of the SP5.2/tTF-OCMCs-SPIO-NPs. These properties separately belong to SP5.2, Fe3O4 and tTF moieties of the SP5.2/tTF-OCMCs-SPIO-NPs. Thus these SP5.2/tTF-OCMCs-SPIO-NPs with double-targeting function should have a potential application in embolization therapy of tumor blood vessels. (C) 2012 Elsevier B. V. All rights reserved.

引用

页码：248 / 255

页数：8

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