Analysis of expression of growth factor receptors in replicatively and oxidatively senescent human fibroblasts

被引：13

作者：

Chen, JH ^{[1
]}

Ozanne, SE ^{[1
]}

Hales, CN ^{[1
]}

机构：

[1] Univ Cambridge, Addenbrookes Hosp, Dept Clin Biochem, Cambridge CB2 2QR, England

来源：

FEBS LETTERS | 2005年 / 579卷 / 28期

基金：

美国国家卫生研究院;

关键词：

senescence; oxidative stress; insulin receptor; growth factor receptor; mitogen;

D O I：

10.1016/j.febslet.2005.09.102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Replicatively and oxidatively senescent human fibroblasts demonstrate an impaired response to mitogens. To investigate whether this is due to downregulation of growth factor receptors we examined their expression in these two types of senescence. mRNA and protein levels of the insulin receptor and platelet-derived growth factor (PDGF) alpha-receptor decreased in replicatively senescent cells. The PDGF beta-receptor and insulin-like growth factor 1 receptor at the protein level also decreased but remained readily detectable. However, these major growth factor receptors remained unchanged in oxidatively premature senescent cells. This suggests that mechanisms underlying diminished responsiveness to mitogens might be different in replicative senescence and oxidatively premature senescence. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：6388 / 6394

页数：7

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