Optimization of Enzymatic Synthesis of Cetyl 2-Ethylhexanoate by Novozym® 435

被引:17
作者
Chen, Hsiao Ching [2 ]
Kuo, Chia-Hung [1 ]
Chen, Hsin Hung [2 ]
Liu, Yung-Chuan [3 ]
Shieh, Chwen Jen [1 ]
机构
[1] Natl Chung Hsing Univ, Ctr Biotechnol, Taichung 402, Taiwan
[2] Dayeh Univ, Dept & Grad Program Bioindustry Technol, Changhua 515, Taiwan
[3] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 402, Taiwan
关键词
Biocatalysis; Cetyl; 2-ethylhexanoate; Lipase; Optimization; Response surface methodology; Wax esters; LIPASE-CATALYZED SYNTHESIS; SURFACE METHODOLOGY RSM; WAX ESTER; WATER-CONTENT; ALCOHOLYSIS; TRIOLEIN; MEDIA; ACID;
D O I
10.1007/s11746-011-1868-y
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Waxes are esters obtained from long-chain fatty acids and long-chain alcohols which are biodegradable, biocompatible and nontoxic. Seafowl feather oil is a natural wax ester that exists on seafowl feathers. Cetyl 2-ethylhexanoate is the major ingredient of seafowl feather oil. Cetyl 2-ethylhexanoate is widely used in cosmetics as a base oil because of its lubricity, moisture retention and non-toxic properties. An optimal production of cetyl 2-ethylhexanoate by direct esterification of cetyl alcohol with 2-ethylhexanoic acid was developed using an immobilized lipase (Novozym(A (R)) 435) as a catalyst in n-hexane. Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) were employed to evaluate the effects of reaction time, reaction temperature, substrate molar ratio, and enzyme amount on the yield of cetyl 2-ethylhexanoate. The results show that reaction time, reaction temperature, substrate molar ratio, and enzyme amount have significant effects on the yield of the esterification reaction. On the basis of ridge-max analysis, the optimum conditions were as follows: a reaction time of 2.65 days, a reaction temperature of 56.18 A degrees C, a substrate molar ratio of 2.55:1, and an enzyme amount of 251.39%. The predicted and experimental values of molar conversion were 91.95 and 89.75 +/- A 1.06%, respectively.
引用
收藏
页码:1917 / 1923
页数:7
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