PvLOX2 silencing in common bean roots impairs arbuscular mycorrhiza-induced resistance without affecting symbiosis establishment

被引：13

作者：

Mora-Romero, Guadalupe A. ^{[1
,2
]}

Gonzalez-Ortiz, Maria A. ^{[1
]}

Quiroz-Figueroa, Francisco ^{[1
]}

Calderon-Vazquez, Carlos L. ^{[1
]}

Medina-Godoy, Sergio ^{[1
]}

Maldonado-Mendoza, Ignacio ^{[1
]}

Arroyo-Becerra, Analilia ^{[3
]}

Perez-Torres, Anahi ^{[4
]}

Alatorre-Cobos, Fulgencio ^{[4
]}

Sanchez, Federico ^{[5
]}

Lopez-Meyer, Melina ^{[1
]}

机构：

[1] Inst Politecn Nacl CIIDIR Sinaloa, Dept Biotecnol Agr, Guasave 81101, Mexico

[2] Univ Occidente, Inst Invest Ambiente & Salud, Los Mochis 81200, Mexico

[3] Inst Politecn Nacl CIBA Tlaxcala, Tlaxcala 90700, Mexico

[4] Ctr Invest & Estudios Avanzados IPN, UGA, Irapuato 36821, Mexico

[5] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Mexico City 62210, DF, Mexico

来源：

FUNCTIONAL PLANT BIOLOGY | 2015年 / 42卷 / 01期

关键词：

fungi; RNA silencing; systemic resistance; DEFENSE GENE RESPONSES; PATHOGEN INFECTION; PHASEOLUS; LIPOXYGENASE; LEAVES; BIOSYNTHESIS; ACCUMULATION; INDUCTION; JASMONATE; FUNGI;

D O I：

10.1071/FP14101

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The arbuscular mycorrhizal (AM) symbiosis is an intimate association between specific soil-borne fungi and the roots of most land plants. AM colonisation elicits an enhanced defence resistance against pathogens, known as mycorrhizal-induced resistance (MIR). This mechanism locally and systemically sensitises plant tissues to boost their basal defence response. Although a role for oxylipins in MIR has been proposed, it has not yet been experimentally confirmed. In this study, when the common bean (Phaseolus vulgaris L.) lipoxygenase PvLOX2 was silenced in roots of composite plants, leaves of silenced plants lost their capacity to exhibit MIR against the foliar pathogen Sclerotinia sclerotiorum, even though they were colonised normally. PvLOX6, a LOX gene family member, is involved in JA biosynthesis in the common bean. Downregulation of PvLOX2 and PvLOX6 in leaves of PvLOX2 root-silenced plants coincides with the loss of MIR, suggesting that these genes could be involved in the onset and spreading of the mycorrhiza-induced defence response.

引用

页码：18 / 30

页数：13