Haploid plant regeneration from unpollinated ovule cultures of niger (Guizotia abyssinica (L. f.) cass.)

被引：3
|
作者：
Bhat, J. G. [1 ]
Murthy, H. N. [1 ]
机构：
[1] Karnatak Univ, Dept Bot, Dharwad 580003, Karnataka, India
来源：
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2008年 / 55卷 / 02期
关键词：
guizotia abyssinica; gynogenesis; haploids; ovule culture; parthenogenesis; plant regeneration;
D O I：
10.1134/S1021443708020118
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Haploid plants were regenerated in vitro from unpollinated ovules of niger (Guizotia abyssinica (L. f.) (Cass.) on Murashige and Skoog nutrient medium (MS) supplemented with 10 mu M naphthaleneacetic acid or 10 mu M NAA + 1.5 mu M kinetin and 30 g/l sucrose. Gamborg (B5) medium was the best for plant regeneration (in comparison with MS, Nitsch and Nitsch (NN), and Chu (N6) media) from cultured ovules, and 6.66 and 7.33 ovules of JNC-6 and Ootacamund cultivars were involved in direct plant regeneration on this medium. Matured ovules (ovules collected one day before anthesis or on the day of anthesis) only responded to cultural regimes and involved in direct plantlet development. Cytological preparation of root tips and chloroplast counts in the guard cells of leaf stomata of regenerated plants confirmed their haploid nature.
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页码：241 / 245
页数：5
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