Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice

被引：6

作者：

Wu, Rui ^{[1
,2
,3
]}

Tian, Yongxiang ^{[1
,2
]}

Deng, Junhua ^{[1
]}

Yang, Keli ^{[1
]}

Liang, Wangwang ^{[1
]}

Guo, Rui ^{[1
]}

Duan, Zhengying ^{[1
]}

Liu, Zewen ^{[1
]}

Zhou, Danna ^{[1
]}

Xu, Diping ^{[1
,2
]}

机构：

[1] Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430064, Hubei, Peoples R China

[2] Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Hubei, Peoples R China

[3] Luoyang Pu Like Bioengn Co Ltd, Natl Engn Res Ctr Anim Drugs, Luoyang 471003, Henan, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2011年 / 156卷 / 04期

关键词：

Japanese encephalitis virus; Swine; Mouse; Adaptation; Pathogenicity; ANTIBODIES; SEQUENCE; PIGS; NS5;

D O I：

10.1007/s00705-010-0871-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In a previous study, we performed serial brain-to-brain passages of swine Japanese encephalitis virus in mice and sequenced the complete genomes of the F5 and F20 passaged mouse-adapted variants. In the current study, we analyzed the differences between their genome sequences and found 12 amino acid substitutions in the nonstructural proteins. We also assessed the growth characteristics of these two variants in mammalian cells in vitro and in vivo. Our investigations revealed that the F20 variant had enhanced growth characteristics and modified virulence compared with the F5 variant. We therefore conclude that multiple amino acid substitutions in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.

引用

页码：685 / 688

页数：4

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