Promoting pepper (Capsicum annuum) photosynthesis via chloroplast ultrastructure and enzyme activities by optimising the ammonium to nitrate ratio

被引:6
作者
Zhang, Jing [1 ]
Xie, Jianming [1 ]
Gan, Yantai [2 ]
Coulter, Jeffrey A. [3 ]
Dawuda, Mohammed Mujitaba [1 ,4 ]
Yu, Jihua [1 ]
Lv, Jian [1 ]
Li, Jing [1 ]
Zhang, Xiaodang [1 ]
Tang, Chaonan [1 ]
Wang, Cheng [1 ]
Niu, Tianhang [1 ]
Calderon-Urrea, Alejandro [5 ,6 ]
机构
[1] Gansu Agr Univ, Coll Hort, Lanzhou 730070, Peoples R China
[2] Agr & Agri Food Canada, Swift Current Res & Dev Ctr, Swift Current, SK S9H 3X2, Canada
[3] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA
[4] Univ Dev Studies, Dept Hort, Fac Agr, POB TL 1882, Tamale, Ghana
[5] Gansu Agr Univ, Coll Plant Protect, Lanzhou 730070, Peoples R China
[6] Calif State Univ Fresno, Dept Biol, Coll Sci & Math, Fresno, CA 93740 USA
关键词
chlorophyll fluorescence; gas exchange parameter; glutamate dehydrogenase; photosynthetic pigment; Rubisco; CHLOROPHYLL FLUORESCENCE CHARACTERISTICS; LIGHT-RESPONSE CURVES; GAS-EXCHANGE; NITROGEN FORM; HIGHER-PLANTS; ANTIOXIDANT SYSTEM; ELECTRON-TRANSPORT; WATER-STRESS; L; PLANTS; GROWTH;
D O I
10.1071/FP19149
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Optimal plant growth in many species is achieved when the two major forms of N are supplied at a particular ratio. In this pot experiment, the effects of five different ammonium : nitrate ratios (ANRs) (0 : 100, 12.5 : 87.5, 25 : 75, 37.5 : 62.5, and 50 : 50) on photosynthesis efficiency in chilli pepper (Capsicum annuum L.) plants were evaluated. The results showed that an ANR of 25 : 75 increased the contents of chl a, leaf area and dry matter, whereas chl b content was not affected by the ANRs. Regarding chlorophyll fluorescence, an ANR of 25 : 75 also enhanced the actual photochemical efficiency, photochemical quenching and maximum photosynthetic rate. However, the 0 : 100 and 50 : 50 ANRs resulted in higher values for nonphotochemical quenching. An inhibition of maximal photochemical efficiency was found when 50% NH4+ was supplied at the later stage of plant growth. The addition of 25% or 37.5% NH4+ was beneficial for gas exchange parameters and the 25% NH4+ optimised the thylakoid of chloroplasts. Compared with nitrate alone, 12.5-50% NH4+ upregulated glutamate dehydrogenase (GDH), the large subunit and the small subunit of Rubisco. It can be concluded that the 25 : 75 ANR accelerated N assimilation through active GDH, which provides a material basis for chloroplast and Rubisco formation, resulting in the increased photosynthetic rate and enhanced growth in chilli pepper.
引用
收藏
页码:303 / 317
页数:15
相关论文
共 71 条
[1]   Effect of chromium and nitrogen form on photosynthesis and anti-oxidative system in barley [J].
Ali, S. ;
Farooq, M. A. ;
Jahangir, M. M. ;
Abbas, F. ;
Bharwana, S. A. ;
Zhang, G. P. .
BIOLOGIA PLANTARUM, 2013, 57 (04) :758-763
[2]  
[Anonymous], 1950, PLANT SOIL
[3]   COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].
ARNON, DI .
PLANT PHYSIOLOGY, 1949, 24 (01) :1-15
[4]   Electron flow to oxygen in higher plants and algae: rates and control of direct photoreduction (Mehler reaction) and rubisco oxygenase [J].
Bader, MR ;
von Caemmerer, S ;
Ruuska, S ;
Nakano, H .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2000, 355 (1402) :1433-1445
[5]   PHOTON YIELD OF O-2 EVOLUTION AND CHLOROPHYLL FLUORESCENCE CHARACTERISTICS AT 77-K AMONG VASCULAR PLANTS OF DIVERSE ORIGINS [J].
BJORKMAN, O ;
DEMMIG, B .
PLANTA, 1987, 170 (04) :489-504
[6]  
Bjorkman O., 1995, Ecophysiology of photosynthesis, P17, DOI [DOI 10.1007/978-3-642-79354-72, 10.1007/978-3-642-79354-7_2, DOI 10.1007/978-3-642-79354-7_2, 10.1007/978-3-642-79354-72]
[7]   Effect of nitrogen form and nutrient solution pH on growth and mineral composition of self-grafted and grafted tomatoes [J].
Borgognone, Daniela ;
Colla, Giuseppe ;
Rouphael, Youssef ;
Cardarelli, Mariateresa ;
Rea, Elvira ;
Schwarz, Dietmar .
SCIENTIA HORTICULTURAE, 2013, 149 :61-69
[8]   NH4+ toxicity in higher plants:: a critical review [J].
Britto, DT ;
Kronzucker, HJ .
JOURNAL OF PLANT PHYSIOLOGY, 2002, 159 (06) :567-584
[9]   Effect of elevated CO2 concentration and nitrate: ammonium ratios on gas exchange and growth of cassava (Manihot esculenta Crantz) [J].
Cruz, Jailson L. ;
Alves, Alfredo A. C. ;
LeCain, Daniel R. ;
Ellis, David D. ;
Morgan, Jack A. .
PLANT AND SOIL, 2014, 374 (1-2) :33-43
[10]   Limitations to photosynthesis in Coffea canephora as a result of nitrogen and water availability [J].
DaMatta, FM ;
Loos, RA ;
Silva, EA ;
Loureiro, ME .
JOURNAL OF PLANT PHYSIOLOGY, 2002, 159 (09) :975-981