Zebrafish foxc1a Plays a Crucial Role in Early Somitogenesis by Restricting the Expression of aldh1a2 Directly

被引:25
作者
Li, Jingyun [1 ,2 ]
Yue, Yunyun [1 ]
Dong, Xiaohua [1 ]
Jia, Wenshuang [1 ]
Li, Kui [1 ]
Liang, Dong [1 ]
Dong, Zhangji [1 ]
Wang, Xiaoxiao [1 ]
Nan, Xiaoxi [1 ]
Zhang, Qinxin [1 ]
Zhao, Qingshun [1 ]
机构
[1] Nanjing Univ, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing 210061, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Maternal & Child Hlth Med Inst, Nanjing Maternal & Child Hlth Care Hosp, Nanjing 210004, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Fibroblast Growth Factor (FGF); MyoD; Retinoic Acid; Transcription Activator-like Effector Nuclease (TALEN); Zebrafish; aldh1a2; deltaC; foxc1a; Paraxial Mesoderm; Somitogenesis; HELIX TRANSCRIPTION FACTORS; RETINOIC ACID CONTROLS; ONE-EYED PINHEAD; SEGMENTATION CLOCK; SOMITE FORMATION; NO TAIL; EMBRYONIC-DEVELOPMENT; WILD-TYPE; IN-VIVO; GENE;
D O I
10.1074/jbc.M114.612572
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Foxc1a is a member of the forkhead transcription factors. It plays an essential role in zebrafish somitogenesis. However, little is known about the molecular mechanisms underlying its controlling somitogenesis. To uncover how foxc1a regulates zebrafish somitogenesis, we generated foxc1a knock-out zebrafish using TALEN (transcription activator-like effector nuclease) technology. The foxc1a null embryos exhibited defective somites at early development. Analyses on the expressions of the key genes that control processes of somitogenesis revealed that foxc1a controlled early somitogenesis by regulating the expression of myod1. In the somites of foxc1a knock-out embryos, expressions of fgf8a and deltaC were abolished, whereas the expression of aldh1a2 (responsible for providing retinoic acid signaling) was significantly increased. Once the increased retinoic acid level in the foxc1a null embryos was reduced by knocking down aldh1a2, the reduced expression of myod1 was partially rescued by resuming expressions of fgf8a and deltaC in the somites of the mutant embryos. Moreover, a chromatin immunoprecipitation assay on zebrafish embryos revealed that Foxc1a bound aldh1a2 promoter directly. On the other hand, neither knocking down fgf8a nor inhibiting Notch signaling affected the expression of aldh1a2, although knocking down fgf8a reduced expression of deltaC in the somites of zebrafish embryos at early somitogenesis and vice versa. Taken together, our results demonstrate that foxc1a plays an essential role in early somitogenesis by controlling Fgf and Notch signaling through restricting the expression of aldh1a2 in paraxial mesoderm directly.
引用
收藏
页码:10216 / 10228
页数:13
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