Electrocatalytic detection of NADH and ethanol at glassy carbon electrode modified with electropolymerized films from methylene green

被引：46

作者：

Dai, Z. -H. ^{[1
]}

Liu, F. -X.

Lu, G. -F.

Bao, J. -C.

机构：

[1] Nanjing Normal Univ, Dept Chem, Nanjing 210097, Peoples R China

[2] Nanjing XiaoZhuang Coll, Dept Chem, Nanjing 210017, Peoples R China

来源：

JOURNAL OF SOLID STATE ELECTROCHEMISTRY | 2008年 / 12卷 / 02期

基金：

中国国家自然科学基金;

关键词：

NADH; methylene green; detection; alcohol dehydrogenase; ethanol; electropolymerization;

D O I：

10.1007/s10008-007-0378-1

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

The oxidation of NADH on electropolymerizing methylene green (MG)-modified glassy carbon electrode (GCE) is described. The modified electrode shows an excellent electrocatalytic activity toward NADH oxidation, reducing its overpotential by about 650 mV and exhibits a wide linear range of 5.6-420 mu M NADH with the detection limit of 3.8 mu M. The electrode displays a good reproducibility and stability and the coexisting species does not affect the determination of NADH. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this electrode. MG-modified GCE can not only be used to detect NADH in biochemical reaction, but also can be used as the potential matrix of the construction of dehydrogenases biosensor.

引用

页码：175 / 180

页数：6

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