Site-specific DNA cleavage by artificial zinc finger-type nuclease with cerium-binding peptide

被引：34

作者：

Nakatsukasa, T ^{[1
]}

Shiraishi, Y ^{[1
]}

Negi, S ^{[1
]}

Imanishi, M ^{[1
]}

Futaki, S ^{[1
]}

Sugiura, Y ^{[1
]}

机构：

[1] Kyoto Univ, Chem Res Inst, Uji, Kyoto 6110011, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 330卷 / 01期

关键词：

zinc finger protein; lanthanide ion; calcium-binding loop; DNA binding; DNA cleavage; artificial nuclease;

D O I：

10.1016/j.bbrc.2005.02.164

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The addition of a new function to native proteins is one of the most attractive protein-based designs. In this study, we have converted a C2H2-type zinc finger as a DNA-binding motif into a novel zinc finger-type nuclease by connecting two distinct zinc finger proteins (Sp1 and GLI) with a functional linker possessing DNA cleavage activity. As a DNA cleavage domain, we chose an analogue of the metal-binding loop (12 amino acid residues), peptide P1, which has been reported to exhibit a strong binding affinity for a lanthanide ion and DNA cleavage ability in the presence of Ce(IV). Our newly designed nucleases, Sp1(PI)GLI and Sp1(P1G)GLI, can strongly bind to a lanthanide ion and show a unique DNA cleavage pattern, in which certain positions between the two DNA-binding sites are specifically cleaved. The present result provides useful information for expanding the design strategy for artificial nucleases. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：247 / 252

页数：6

共 31 条

[1]

CAZQUEZIBAR JL, 2002, P NATL ACAD SCI USA, V99, P3487

[2] Site-selective DNA hydrolysis by combining Ce(IV)/EDTA with monophosphate-bearing oligonucleotides and enzymatic ligation of the scission fragments [J].

Chen, W ;

Kitamura, Y ;

Zhou, JM ;

Sumaoka, J ;

Komiyama, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (33) :10285-10291

[3] Circular dichroism spectra of short, fixed-nucleus alanine helices [J].

Chin, DH ;

Woody, RW ;

Rohl, CA ;

Baldwin, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) :15416-15421

[4] SELECTION OF DNA-BINDING SITES FOR ZINC FINGERS USING RATIONALLY RANDOMIZED DNA REVEALS CODED INTERACTIONS [J].

CHOO, Y ;

KLUG, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :11168-11172

[5] TOWARD A CODE FOR THE INTERACTIONS OF ZINC FINGERS WITH DNA - SELECTION OF RANDOMIZED FINGERS DISPLAYED ON PHAGE [J].

CHOO, Y ;

KLUG, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :11163-11167

[6] LUMINESCENCE OF PEPTIDE-BOUND TERBIUM IONS - DETERMINATION OF BINDING CONSTANTS [J].

DADLEZ, M ;

GORAL, J ;

BIERZYNSKI, A .

FEBS LETTERS, 1991, 282 (01) :143-146

[7] Development of zinc finger domains for recognition of the 5′-ANN-3′ family of DNA sequences and their use in the construction of artificial transcription factors [J].

Dreier, B ;

Beerli, RR ;

Segal, DJ ;

Flippin, JD ;

Barbas, CF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (31) :29466-29478

[8] CONVERSION OF A HELIX-TURN-HELIX MOTIF SEQUENCE-SPECIFIC DNA-BINDING PROTEIN INTO A SITE-SPECIFIC DNA CLEAVAGE AGENT [J].

EBRIGHT, RH ;

EBRIGHT, YW ;

PENDERGRAST, PS ;

GUNASEKERA, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :2882-2886

[9] INCORPORATION OF AN EDTA-METAL COMPLEX AT A RATIONALLY SELECTED SITE WITHIN A PROTEIN - APPLICATION TO EDTA-IRON DNA AFFINITY CLEAVING WITH CATABOLITE GENE ACTIVATOR PROTEIN (CAP) AND CRO [J].

EBRIGHT, YW ;

CHEN, Y ;

PENDERGRAST, PS ;

EBRIGHT, RH .

BIOCHEMISTRY, 1992, 31 (44) :10664-10670

[10] Zif268 protein-DNA complex refined at 1.6 angstrom: A model system for understanding zinc finger-DNA interactions [J].

ElrodErickson, M ;

Rould, MA ;

Nekludova, L ;

Pabo, CO .

STRUCTURE, 1996, 4 (10) :1171-1180

← 1 2 3 4 →