Enhanced Herbicide Metabolism and Metabolic Resistance Genes Identified in Tribenuron-Methyl Resistant Myosoton aquaticum L.

被引：37

作者：

Bai, Shuang ^{[1
]}

Liu, Weitang ^{[1
]}

Wang, Hengzhi ^{[1
]}

Zhao, Ning ^{[1
]}

Jia, Sisi ^{[2
]}

Zou, Nan ^{[1
]}

Guo, Wenlei ^{[3
]}

Wang, Jinxin ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Plant Protect, Key Lab Pesticide Toxicol & Applicat Tech, Tai An 271018, Shandong, Peoples R China

[2] Taian Customs, Tai An 271000, Shandong, Peoples R China

[3] Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou 510640, Guangdong, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 37期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

abiotic stress; Myosoton aquaticum L; acetolactate synthase; tribenuron-methyl; metabolic resistance; transcriptomics; molecular mechanisms; SITE-BASED RESISTANCE; CYTOCHROME-P450 MONOOXYGENASE CDNA; ACETOLACTATE SYNTHASE INHIBITORS; ARABIDOPSIS; TARGET; WHEAT; EXPRESSION; EVOLUTION; ALS; SUPERFAMILY;

D O I：

10.1021/acs.jafc.8b02740

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The evolved resistance of Myosoton aquaticum L. to acetolactate synthase (ALS) inhibitors is well established, but most research has focused on target-site resistance, while nontarget-site resistance remains neglected. Here, we investigated mechanisms of the latter. The pretreatment with the P450 inhibitor malathion significantly increased the sensitivity of resistant plants to tribenuron-methyl. The rapid P450-mediated tribenuron-methyl metabolism in resistant plants was confirmed by LC-MS/MS analysis. Besides, GST activity was higher among resistant than susceptible individuals. The next transcriptome analysis generated 544,102,236 clean reads from RNA sequencing libraries. De novo assembly yielded 102,529 unigenes with an average length of 866 bp, annotated across seven databases. Digital gene expression selected 25 differentially expressed genes, further validated with qRT-PCR Three P450 genes, two GST genes, two glucosyltransferase genes, four ABC transporter genes, and four additional contigs were constitutively up-regulated in resistant individuals. Overall, our research confirmed that enhanced herbicide metabolism drives tribenuron-methyl resistance in M. aquaticum.

引用

页码：9850 / 9857

页数：8

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