An analytical method for determining relative specificities for sequential reactions catalyzed by the same enzyme:: Application to the hydrolysis of triacylglycerols by lipases

被引：15

作者：

Mitchell, David Alexander ^{[1
]}

Rodriguez, Jorge A. ^{[2
]}

Carriere, Frederic ^{[2
]}

Baratti, Jacques ^{[3
]}

Krieger, Nadia ^{[4
]}

机构：

[1] Univ Fed Parana, Dept Bioquim & Biol Mol, Ctr Politecn, BR-81531990 Curitiba, Parana, Brazil

[2] CNRS, Lab Enzymol Interfaciale & Physiol Lipolyse, UPR 9025, Marseille, France

[3] Univ Aix Marseille 2, Grp Biocatalyse & Chimie Fine, Marseille, France

[4] Univ Fed Parana, Dept Quim, Ctr Politecn, BR-81531990 Curitiba, Parana, Brazil

来源：

JOURNAL OF BIOTECHNOLOGY | 2008年 / 133卷 / 03期

关键词：

lipase; mathematical model; specificity constant; triacylglycerol; diacylglycerol; monoacylglycerol; sequential reactions;

D O I：

10.1016/j.jbiotec.2007.10.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We propose a model for the sequential hydrolysis of ester bonds of triacylglycerols by lipases and use it as the basis for an analytical method for determining the relative specificity of the lipase for the various substrates with which it can react, when the substrates occur simultaneously in a single reaction system. We then apply the method to our own data and literature data involving the hydrolysis of triacylglycerols by lipases. Our model is able to fit well to most of the reaction profiles, enabling the estimation of relative specificities. We discuss the limitations and potential applications of our method. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：343 / 350

页数：8

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