Associations between LEP, DGAT1 and FABP4 gene polymorphisms and carcass and meat traits in Nelore and crossbred beef cattle

被引：16

作者：

Curi, R. A. ^{[1
]}

Chardulo, L. A. L. ^{[2
]}

Arrigoni, M. D. B. ^{[1
]}

Silveira, A. C. ^{[1
]}

de Oliveira, H. N. ^{[3
]}

机构：

[1] Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Melhoramento & Nutr Anim, BR-18618000 Botucatu, SP, Brazil

[2] Univ Estadual Paulista, Inst Biociencias, Dept Quim & Bioquim, BR-18618000 Botucatu, SP, Brazil

[3] Univ Estadual Paulista, Fac Ciencias Agrarias & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, Brazil

来源：

LIVESTOCK SCIENCE | 2011年 / 135卷 / 2-3期

基金：

巴西圣保罗研究基金会;

关键词：

Molecular markers; Candidate gene; Fat deposition; Meat tenderness; Zebu; COA-DIACYLGLYCEROL ACYLTRANSFERASE; SINGLE NUCLEOTIDE POLYMORPHISM; MISSENSE MUTATION; VARIABLE NUMBER; FAT DEPOSITION; DAIRY-CATTLE; BOS-INDICUS; MILK-YIELD; BOVINE; LOCUS;

D O I：

10.1016/j.livsci.2010.07.013

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aim of this study was to estimate the segregation of leptin (LEP), diacylglycerol O-acyltransferase (DGAT1) and fatty acid-binding protein 4 (FABP4) gene polymorphisms in Nelore (Bos indicus) and Nelore x Bos taurus beef cattle, and to evaluate their effects on carcass and meat traits. A total of 300 animals were genotyped for the LEP/BsaAl (Y_11369.1: g.1620G > A), DGAT1-VNTR (18-bp repeat element in the promoter region) and FABP4/MspA1I (AAFC_01136716.1:g.7516C > C) polymorphisms and phenotyped for rib eye area, backfat thickness (BT), intramuscular fat, shear force, and myofibrillar fragmentation index. The allele substitution effects for each of the polymorphisms on the traits of interest were estimated by regression on the number of copies of a particular allele using the General Linear Model procedure. To keep the experiment wise error rate to the specified level, the Bonferroni adjustment was applied. Although the LEP/BsaAl polymorphism has shown segregation for association studies in Nelore cattle [f(A) = 0.351], no associations were observed between its alleles and the traits analyzed. The DGAT1-VNTR was found to be polymorphic in Nelore cattle, as well as a potential association with BT. In addition to being non-informative in Nelore animals (allele C was found to be fixed), the FABP4/MspA1I polymorphism showed no association with the studied traits in crossbred animals. (c) 2010 Elsevier B.V. All rights reserved.

引用

页码：244 / 250

页数：7

共 36 条

[1] The leptin C73T missense mutation is not associated with marbling and fatness traits in a large gene mapping experiment in Australian cattle [J].

Barendse, W ;

Bunch, RJ ;

Harrison, BE .

ANIMAL GENETICS, 2005, 36 (01) :86-88

[2] A splice site single nucleotide polymorphism of the fatty acid binding protein 4 gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle [J].

Barendse, W. ;

Bunch, R. J. ;

Thomas, M. B. ;

Harrison, B. E. .

ANIMAL GENETICS, 2009, 40 (05) :770-773

[3] The DGAT1 K232A mutation is not solely responsible for the milk production quantitative trait locus on the bovine chromosome 14 [J].

Bennewitz, J ;

Reinsch, N ;

Paul, S ;

Looft, C ;

Kaupe, B ;

Weimann, C ;

Erhardt, G ;

Thaller, G ;

Kühn, C ;

Schwerin, M ;

Thomsen, H ;

Reinhardt, F ;

Reents, R ;

Kalm, E .

JOURNAL OF DAIRY SCIENCE, 2004, 87 (02) :431-442

[4]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[5]

Buchanan FC, 2002, GENET SEL EVOL, V34, P105, DOI [10.1051/gse:2001006, 10.1186/1297-9686-34-1-105]

[6] Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis [J].

Cases, S ;

Smith, SJ ;

Zheng, YW ;

Myers, HM ;

Lear, SR ;

Sande, E ;

Novak, S ;

Collins, C ;

Welch, CB ;

Lusis, AJ ;

Erickson, SK ;

Farese, RV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (22) :13018-13023

[7]

Cattle QTL Database, 2009, CATTLE QUANTITATIVE

[8] DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle [J].

Choudhary, Vivek ;

Kumar, Pushpendra ;

Bhattacharya, Tarun K. ;

Bhushan, Bharat ;

Sharma, Arjava .

GENETICS AND MOLECULAR BIOLOGY, 2005, 28 (04) :740-742

[9] RELATIONSHIP OF MYOFIBRIL FRAGMENTATION INDEX TO CERTAIN CHEMICAL, PHYSICAL AND SENSORY CHARACTERISTICS OF BOVINE LONGISSIMUS MUSCLE [J].

CULLER, RD ;

PARRISH, FC ;

SMITH, GC ;

CROSS, HR .

JOURNAL OF FOOD SCIENCE, 1978, 43 (04) :1177-1180

[10]

CURI P. R., 1981, CIENC CULT, V33, P712

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