The Mobilome of Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates

The mobilome structure of the extended spectrum beta-lactamases CTX-M-15 and OXA-48 carbapenemases producing Klebsiella pneumoniae clinical isolates an epidemically common sequence type 395 is characterized. According to the in silico analysis in the mobilome of all strains, the presence of a Col-like plasmid and replicons of the R and L/M incompatibility groups is established. The genetic regions surrounding bla(OXA-48) and bla(CTX-M-15) genes are analyzed and the variants of mobile structures involved in the distribution of these genes are described. In the mobilome structure of K. pneumoniae strain KP1083, an additional plasmid HI1B/FIB is found, whose nucleotide sequence has a phylogenetic relationship to the plasmid pNDM-MAR, as well as a wide range of mobile structures linked to antibiotic resistance determinants, in particular, insertion structures ISCR1 and two integrases related to class 1 integrons.