The detection of feline coronaviruses in blood samples from cats by mRNA RT-PCR

被引：39
作者：
Can-Sahna, Kezban
Ataseven, Veysel Soydal
Pinar, Dilek
Oguzoglu, Tuba Cigdem [1 ]
机构：
[1] Ankara Univ, Fac Vet Med, Dept Virol, TR-06100 Ankara, Turkey
[2] Firat Univ, High Sch Hlth Sci, Elazig, Turkey
[3] Univ Mustafa Kemal, Fac Vet Med, Dept Virol, Antakya, Turkey
来源：
JOURNAL OF FELINE MEDICINE AND SURGERY | 2007年 / 9卷 / 05期
关键词：
D O I：
10.1016/j.jfms.2007.03.002
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In this study, 26 blood samples were collected from 25 healthy cats and one cat with clinical signs suggestive of feline infectious peritonitis (FIP), namely, fever, weight loss, enlarged abdomen, and ascites. Blood samples were tested for feline coronavirus (FCoV) messenger RNA (mRNA) by an reverse transcriptase-polymerase chain reaction (RT-PCR) assay which has previously been described to have a high specificity in the diagnosis of clinical FlP [Simons AF, Vennema H, Rofina JE, Pol JM, Horzinek MC, Rottier PJM,Egberink HF (2005) A mRNA PCR for the diagnosis of feline infectious peritonitis. Journal of Virological Methods 124, 111-116]. Overall we found 14 (54%) of the cats were positive for FCoV including the cat with clinical disease, but the high rate of positivity among healthy cats suggested a poor specificity for the clinical (diagnosis of FlP among these cats. it was observed that the positivity rate was highest in cats aged between 6 months-1year old. Our findings suggest that FCoVs may be present in the blood samples from healthy cats as well as cats with clinical FIR (c) 2007 ESFM and AAFP. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：369 / 372
页数：4
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