Human macrophage tumor necrosis factor (TNF)-α production induced by Trypanosoma brucei gambiense and the role of TNF-α in parasite control

被引:41
作者
Daulouède, S
Bouteille, B
Moynet, D
De Baetselier, P
Courtois, P
Lemesre, JL
Buguet, A
Cespuglio, R
Vincendeau, P
机构
[1] Univ Bordeaux 2, Parasitol Lab, F-33076 Bordeaux, France
[2] Fac Med, Inst Epidemiol Neurol & Neurol Trop, Limoges, France
[3] IRD, Lab Biol Parasitaire, Montpellier, France
[4] CRSSA, Unite Physiol Vigilance, La Tronche, France
[5] INSERM, U480, Dept Expt Med, F-69008 Lyon, France
[6] Inst Mol Biol, Unit Cellular Immunol, St Genesius Rode, Belgium
关键词
D O I
10.1086/319257
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Trypanosoma brucei gambiense, a causative agent of sleeping sickness, induced a dose-dependent production of tumor necrosis factor (TNF)-alpha by human macrophages in vitro. TNF-alpha was also induced in the Mono Mac 6 cell line, which indicates a direct effect of parasite components on macrophages. Parasite-soluble factors were also potent inducers of TNF-alpha. The addition of anti-TNF-alpha to cocultures of macrophages and parasites increased the number of trypanosomes and their life span, whereas irrelevant antibodies had no effect. TNF-alpha may have a direct role (i.e., direct trypanolytic activity) and/or an indirect one, such as TNF alpha -mediated induction of cytotoxic molecules. A direct dose-dependent lytic effect of TNF-alpha on purified parasites was observed. This lytic effect was inhibited by anti-TNF-alpha. These data suggest that, as in experimental trypanosomiasis, TNF-alpha is involved in parasite growth control in human African trypanosomiasis.
引用
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页码:988 / 991
页数:4
相关论文
共 15 条
[1]  
BATE CAW, 1989, IMMUNOLOGY, V66, P600
[2]   THE ROLE OF CACHECTIN/TNF IN ENDOTOXIC-SHOCK AND CACHEXIA [J].
CERAMI, A ;
BEUTLER, B .
IMMUNOLOGY TODAY, 1988, 9 (01) :28-31
[3]   IMMUNOPATHOLOGY OF EXPERIMENTAL AFRICAN SLEEPING SICKNESS - DETECTION OF CYTOKINE MESSENGER-RNA IN THE BRAINS OF TRYPANOSOMA-BRUCEI-BRUCEI-INFECTED MICE [J].
HUNTER, CA ;
GOW, JW ;
KENNEDY, PGE ;
JENNINGS, FW ;
MURRAY, M .
INFECTION AND IMMUNITY, 1991, 59 (12) :4636-4640
[4]   MAPPING THE LECTIN-LIKE ACTIVITY OF TUMOR-NECROSIS-FACTOR [J].
LUCAS, R ;
MAGEZ, S ;
DELEYS, R ;
FRANSEN, L ;
SCHEERLINCK, JP ;
RAMPELBERG, M ;
SABLON, E ;
DEBAETSELIER, P .
SCIENCE, 1994, 263 (5148) :814-817
[5]   Specific uptake of tumor necrosis factor-alpha is involved in growth control of Trypanosoma brucei [J].
Magez, S ;
Geuskens, M ;
Beschin, A ;
delFavero, H ;
Verschueren, H ;
Lucas, R ;
Pays, E ;
deBaetselier, P .
JOURNAL OF CELL BIOLOGY, 1997, 137 (03) :715-727
[6]   Tumor necrosis factor alpha is a key mediator in the regulation of experimental Trypanosoma brucei infections [J].
Magez, S ;
Radwanska, M ;
Beschin, A ;
Sekikawa, K ;
De Baetselier, P .
INFECTION AND IMMUNITY, 1999, 67 (06) :3128-3132
[7]  
Magez S, 1998, J IMMUNOL, V160, P1949
[8]   CORRELATION OF HIGH SERUM LEVELS OF TUMOR-NECROSIS-FACTOR-ALPHA WITH DISEASE SEVERITY IN HUMAN AFRICAN TRYPANOSOMIASIS [J].
OKOMOASSOUMOU, MC ;
DAULOUEDE, S ;
LEMESRE, JL ;
NZILAMOUANDA, A ;
VINCENDEAU, P .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1995, 53 (05) :539-543
[9]  
OLSON T, 1991, EUR J IMMUNOL, V21, P2447
[10]   Endotoxins in the blood and cerebrospinal fluid of patients with African sleeping sickness [J].
Pentreath, VW ;
Alafiatayo, RA ;
Crawley, B ;
Doua, F ;
Oppenheim, BA .
PARASITOLOGY, 1996, 112 :67-73