Purification and characterization of 6-phospho-beta-galactosidase from Lactobacillus gasseri JCM 1031

被引：11
作者：
Suzuki, M [1 ]
Saito, T [1 ]
Itoh, T [1 ]
机构：
[1] TOHOKU UNIV, COLL AGR, DEPT APPL BIOSCI, LAB ANIM PROD CHEM, SENDAI, MIYAGI 981, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 01期
关键词：
6-phospho-beta-galactosidase; Lactobacillus gasseri; purification;
D O I：
10.1271/bbb.60.139
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
6-Phospho-beta-galactosidase from Lactobacillus gasseri JCM 1031 was purified from lactobacilli to homogeneity, about 118-fold, with 0.5% recovery by several chromatographies. The molecular mass and isoelectric point of the purified enzyme were 58 kDa and pI 5.5, respectively, The K-m and V-max for o-nitrophenyl-beta-D-galactopyranoside-6-phosphate mere 0.8 mM and 116.4 mu mol/min/mg, respectively. Reducing agent, Fe2+ ion, and EDTA activated but PCMB, Zn2+, and Hg2+ ions strongly inhibited the enzymatic activity.
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页码：139 / 141
页数：3
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