Participation of singlet oxygen in ultraviolet-a-induced lipid peroxidation in mouse skin and its inhibition by dietary β-carotene:: An ex vivo study

被引:39
作者
Bando, N
Hayashi, H
Wakamatsu, S
Inakuma, T
Miyoshi, M
Nagao, A
Yamauchi, R
Terao, J
机构
[1] Univ Tokushima, Grad Sch Nutr & Biosci, Dept Food Sci, Tokushima 7708503, Japan
[2] Kagome Co, Kagome Res Inst, Tochigi 3292762, Japan
[3] Natl Food Res Inst, Food Mat Div, Tsukuba, Ibaraki 3058642, Japan
[4] Gifu Univ, Fac Agr, Dept Bioproc, Gifu 5011193, Japan
关键词
UV-A; beta-carotene; singlet molecular oxygen; photooxidation; skin damage; free radicals;
D O I
10.1016/j.freeradbiomed.2004.09.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dietary beta-carotene acts as a photoprotective agent in the skin, but the exact mechanism of protection is unknown. This ex vivo study is focused on determining the mechanism of action of beta-carotene against U-V-A-induced skin damage by characterizing peroxidized phosphatidylcholine (PC) and beta-carotene oxidation products. BALB/c mice were fed with basal or a beta-carotene-supplemented diet, and homogenates from their dorsal skin were prepared after 3 weeks for UV-A irradiation. Analyses revealed that the degree of lipid peroxidation in the beta-carotene group was significantly lower than that in the controls. The isomeric composition of hydroperoxy fatty acids, constituting peroxidized PC, was determined by thin-layer chromatography-blotting followed by gas chromatography/mass spectrometry (MS)/selected ion monitoring analysis. The 9- and 10-isomers of peroxidized PC, resulting from the reaction of singlet molecular oxygen (O-1(2)) With oleic acid, were elevated in the UV-A-exposed control group compared to the experimental group. Similar results were obtained from methylene-blue-sensitized photooxidation of mouse skin lipids in vitro. Liquid chromatography/MS analysis of the homogenates confirmed the formation of beta-carotene 5,8-endoperoxide, a specific marker for the O-1(2) reaction. These results indicate that dietary beta-carotene accumulates in the skin and acts as a protective agent against U-V-A-induced oxidative damage, by quenching the O-1(2). (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:1854 / 1863
页数:10
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