Evaluation of a nine-locus variable-number tandem-repeat scheme for typing of Pseudomonas aeruginosa

被引：45

作者：

Turton, J. F. ^{[1
]}

Turton, S. E. ^{[1
]}

Yearwood, L. ^{[1
]}

Yarde, S. ^{[1
]}

Kaufmann, M. E. ^{[1
]}

Pitt, T. L. ^{[1
]}

机构：

[1] Hlth Protect Agcy, Ctr Infect, Lab HealthCare Associated Infect, London NW9 5EQ, England

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2010年 / 16卷 / 08期

关键词：

CYSTIC-FIBROSIS PATIENTS; MULTIPLEX PCR; STRAINS; IDENTIFICATION; OXACILLINASE; OUTBREAK;

D O I：

10.1111/j.1469-0691.2009.03049.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

P>Repeat numbers at nine variable-number tandem-repeat (VNTR) loci were determined for 177 isolates of Pseudomonas aeruginosa representing 77 strains distinguished by pulsed-field gel electrophoresis (PFGE). Eight loci provided for discrimination similar to that provided by PFGE, with variation at the ninth locus (ms61) sometimes allowing discrimination within a PFGE-defined type. The Liverpool and Midlands 1 strains, which are common among patients with cystic fibrosis in the UK, could be unambiguously identified by their characteristic VNTR profiles. In rare cases, the repeat number at the ninth locus alone provided discrimination among isolates that were distinct according to PFGE. In each case, the two isolates shared the same bla(OXA-50-like) allele and belonged to the same oprD sequence type group, supporting the VNTR results in suggesting that they are similar.

引用

页码：1111 / 1116

页数：6

共 12 条

[11] Use of sequence-based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumannii
Turton, J. F.
Gabriel, S. N.
Valderrey, C.
Kaufmann, M. E.
Pitt, T. L.
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (08) : 807 - 815
[12] Multiple-locus variable-number tandem-repeat analysis for longitudinal survey of sources of Pseudomonas aeruginosa infection in cystic fibrosis patients
Vu-Thien, Hoang
Corbineau, Gaelle
Hormigos, Katia
Fauroux, Brigitte
Corvol, Harriet
Clement, Annick
Vergnaud, Gilles
Pourcel, Christine
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (10) : 3175 - 3183

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